兔蛛网膜下腔出血后海马CA_1区微血管形态定量研究及尼莫通的干预作用  

The Study on Microvascular Morphometriology and Effects of Nimotop Treatment in the CA_1 Region of Hippocampal following Subarachnoid Hemorrhage in Rabbits.

作　　者：罗国君[1] 席刚明[2] 范华燕[1] 叶天雄[1] 王云甫[1] 

机构地区：[1]郧阳医学院附属太和医院神经科,湖北省十堰市442000 [2]郧阳医学院附属太和医院神经疾病研究所,湖北省十堰市442000

出　　处：《中国微循环》2001年第2期100-102,共3页Journal of Chinese Microcirculation

摘　　要：目的定量研究兔蛛网膜下腔出血(SAH)后海马CA 1 区微血管形态学变化及相应的神经元病理改变 ,并观察尼莫通 (ND)的治疗作用。方法采用枕大池两次注血法制作兔SAH模型 ,运用内源性过氧化物酶法显示兔SAH后5d海马CA 1 区微血管 ,用体视学方法对脑微血管进行定量分析 ,并观察了海马CA1 区组织病理学变化。结果SAH后海马CA 1 区微血管除直径外 ,体积密度、长度密度、表面积密度较正常组均有明显下降 (P<0 .01) ,且存在神经元的变性坏死 ;ND治疗后海马CA 1 区微血管密度恢复正常 ,与SAH组比较有显著性差异 ,神经元数量显著增加 (P<0.01)。结论SAH后微循环障碍是继发性脑损伤的病理学基础 ;ND具有改善微循环 ,保护神经元的作用。Objectve To evaluate the microvascular morphological chagnges and the protective effects of nimotop treatment in the CA 1 region of hippocampal following subarachnoid hemorrhage(SAH). Methods Experimental SAH in rabbits was made by injecting autologus arterial blood into the cisterna magna twice at 48 hour intervals. Morphometric measurements of the microvascular stained with intrinsic overoxidase in the CA 1 region were performed using stereological analysis system. The pathological change in the CA 1 region was observed. Results Morphological parameters including volume, surface and length densities of the SAH group decreased significantly compared with normal group(P<0.01).Nimotop alleviated all of above pathological alterations. Conclusion The disturbance of cerebral microcirculation following SAH was pathological base of secondary cerebral damage. Nimotop proved to be beneficial in improving microcirculation and protecting nurons.

关 键 词：蛛网膜下腔出血 微血管形态计量学 尼莫通 干预作用 

分 类 号：R743.35[医药卫生—神经病学与精神病学]