胆囊癌细胞系GBC-SD的生物学行为研究  

Study of Human Gallbladder Carcinoma Cell Line on Its Construction and Biological Behavior

作　　者：刘博[1] 王占民[1] 吴小鹏[1] 刘春生[1] 李兆亭[1] 刘军[1] 

机构地区：[1]山东大学齐鲁医院普外科,250012

出　　处：《外科理论与实践》2001年第3期146-148,共3页Journal of Surgery Concepts & Practice

基　　金：山东省自然科学基金;课题编号Y96C13045

摘　　要：目的：建立人体胆囊癌细胞系，研究胆囊癌细胞的生物学行为。方法：应用组织块法建立细胞系，通过细胞形态学、增殖动力学、染色体分析、细胞上清液ＣＥＡ、ＣＡ１９－９检测及异种种植等对其生物行为进行研究。结果：ＧＢＣ－ＳＤ细胞以方形、多角形和梭形为主，倍增时间约２１．４ｈ，染色体３７～１４５条，众数８１条，具维持分泌ＣＥＡ、ＣＡ１９－９的功能，裸鼠种植肿瘤与来源肿瘤组织类型相似。结论：ＧＢＣ－ＳＤ是一新的人体胆囊癌细胞系，可作为研究胆囊癌的实验模型。Objective: To construct a human gallbladder carcinoma cell line and to investigate its biological behavior. Methods: GBC-SD, a human gallbladder carcinoma cell line was constructed via the tissue culture. The biological behavior such as cell morphology, cytokinetics, CEA and CA-199 secretion and growth pattern in heteroimplantation were studied in vitro and in vivo respectively. Results: The GBC-SD cells were mainly composed of square, polygonal and spindal cells, the doubling time was 21.4h. The cell line could secret CEA and CA-199 continuously. The histological features of implanted tumor in nude mice were similar to those of the original one. Conclusions: Being a new constructed gallbladder carcinoma cell line, the GBC-SD cell line could be served as an experimental model for further study.

关 键 词：胆囊肿瘤 细胞系 异种种植 GBC-SD 生物学行为 

分 类 号：R735.8[医药卫生—肿瘤]