鲁氏酵母菌WY-3降解甲胺磷的性能  

Degradation of Methamidophos by Saccharomyces rouxii WY-3

作　　者：刘斌斌[1] 赵永芳[2] 钞亚鹏[1] 谢裕敏[1] 王银善[1] 

机构地区：[1]中国科学院武汉病毒研究所,武汉430071 [2]武汉大学生命科学学院,武汉430072

出　　处：《环境科学》2001年第4期37-41,共5页Environmental Science

基　　金：国家自然科学基金资助项目 !(396 70 0 10 )

摘　　要：从污水中分离出一株酵母菌 ,编号WY 3 .该菌能利用甲胺磷 (MAP)作唯一氮、磷源生长 ,也能利用甲胺、乙胺和硫酸铵 (硝酸盐和羟胺除外 )作氮源生长 ,它还能适应高浓度 (60 % )葡萄糖的培养条件 ,初步鉴定为鲁氏酵母菌 (Saccharomycesrouxii) .从WY 3菌无细胞抽提液中检测到一种高活力酸性磷酸酶 ,采用聚丙烯酰胺凝胶电泳活性染色法呈现一条白色谱带 .氟化钠试剂可抑制该酶催化MAP释放无机磷 ,但是不影响该菌代谢MAP脱氨基的性能 .MAP经WY 3菌作用后毒性会大大降低 ,分析其残留物时发现有无机磷和甲醇物质 .此外 。A strain of Yeast WY\|3 was isolated from wastewater sample.It is capable of utilizing methamidophos as sole nitrogen and phosphorus sources,and also capable of utilizing methylamine,ethylamine and ammonium sulfate as nitrogen sources except nitrate and hydroxylamine.The yeast could grow in medinm containing 60% glucose and was identified as \%Saccharomyces rouxii\% WY\|3.The strain contains a high active acid phosphatase.The crude enzyme was applied to a plate of polyacrylanide gel for electrophoresis,then activity was detected as white single band.Inhibiting test showed that sodium fluoride could seriously inhibit the activity of acid phosphatase to release phosphorus from methamidophos,wherease it make no effect on deamination of the strain WY\|3 from methamidophos.After methamidophos was degraded by strain WY\|3,toxity of the pesiticide reduced obviously,and its portion intermediate,methanol and inorganic phosphorus,were deteted.

关 键 词：鲁氏酵母菌 甲胺磷 酸性磷酸酶 降解 废水处理 农药 WY-3 

分 类 号：X172[环境科学与工程—环境科学] X786