糖化肌醇磷脂结合蛋白在健康人淋巴细胞及其亚群的表达  

Expression of glycosylphosphatidylinositol-anchored protein in lymphocytes and the subsets of healthy individuals

作　　者：崔巍[1] 杨敏[2] 张之南[3] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院检验科,北京100730 [2]北京同仁医院 [3]中国医学科学院中国协和医科大学北京协和医院血液科,北京100730

出　　处：《中华检验医学杂志》2001年第3期158-160,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的　研究健康人淋巴细胞及其亚群上 ,糖化肌醇磷脂结合蛋白 (GPI锚蛋白 )之一CD5 9的表达 ,探讨淋巴细胞及其亚群能否做为诊断阵发性睡眠性血红蛋白尿症的指标。方法　采用流式细胞术多色分析法 ,结合多种细胞特异性抗体 ,分析淋巴细胞及其亚群和中性粒细胞上CD5 9的表达情况。结果　 5 0名健康人中性粒细胞上CD5 9的表达均为完全阳性 ,表达率为 (98 6± 1 5 ) %。淋巴细胞上CD5 9的表达率为 (90 0± 14 9) % ,少量呈现阴性和不完全阳性表达。CD3+ T细胞与淋巴细胞类似 ,但完全阳性细胞少于后者 ,部分阳性和阴性细胞多于后者。与CD3+ T细胞相比 ,CD4+ T细胞上CD5 9的表达率增高 ,为 (92 9± 8 1) % ,完全阳性细胞增多 ,但仍有少量阴性细胞存在 ;CD8+ T细胞表达率明显下降 ,为 (74 6± 9 1) % ,完全阳性细胞减少 ,部分阳性和阴性细胞增多 ;CD45RA+ 和CD45RO+ T细胞的表达差异无显著意义 (P >0 0 5 ) ;活化T细胞的表达介于CD3+ 和CD8+ T细胞之间。B细胞的表达均为完全阳性 ,表达率高达 (96 3± 0 .6 ) % ,与中性粒细胞类似。结论　健康人淋巴细胞上CD5 9不完全阳性的表达主要与T细胞、尤其是CD8+ T细胞有关 ;B细胞表达均一 。Objective To study the expression of glycosylphosphatidylinositol(GPI) anchored protein in lymphocyte subsets of healthy individuals. Methods Flow cytometry and two/three color McAbs were used to detect CD59 expression in granulocytes, lymphocytes and subsets. Results In 50 samples, granulocytes mainly showed a single population of cells strongly positive for CD59, the percentage of CD59 expression was (98.6±1 5)%. Lymphocytes had a small population of weakly positive and negative for CD59, positive cells being (90 0± 14.9)%. CD3 +T cells had a similar profile as that of total lymphocytes, but there were more weakly positive /negative cells and less strong positive cells. As compared with CD3 +T cells, CD4 +T cells had a larger population of strongly positive for CD59, positive cells being (92.9±8.1)%; while CD8 +T cells had a smaller population of strongly positive, positive cells being (74.6±9.1)%. CD59 expression of activated T cells was between that of CD3 +and CD8 +T cells. CD45RA +and CD45RO +T cells both had a similar profile like that of CD3 +T cells. CD20 +B cells showed a similar profile to that of granulocytes, positive cells being (96.3±0 6)%. Conclusions Population of low or negative expression of CD59 among the lymphocytes of healthy controls was related to T cells, especially CD8 +T cells. B cells had uniform positive expression of CD59, and could be a candidate for detecting PNH.

关 键 词：淋巴细胞亚群 糖基磷脂酰肌醇类 载体蛋白质类 阵发性睡眠性血红蛋白尿症 GPI锚蛋白 

分 类 号：R446.1[医药卫生—诊断学]