肿瘤坏死因子相关凋亡诱导配体受体在肾癌中的分布及其意义  

Expression of tumor necrosis factor-related apoptosis-inducing ligand receptors in renal cell carcinoma

作　　者：张志超[1] 张智清[2] 张强[1] 张立国[2] 曾革非[2] 郭应禄[1] 

机构地区：[1]北京大学第一医院泌尿外科研究所,100034 [2]中国预防医学科学院病毒所

出　　处：《中华外科杂志》2001年第5期375-377,共3页Chinese Journal of Surgery

摘　　要：目的　研究肿瘤坏死因子相关诱导凋亡配体 (TRAIL)受体在肾癌组织中的分布及其意义。　方法　采用RT PCR及NorthernBlot的方法检测TRAIL受体在肾癌组织及正常肾组织 ,肾癌细胞系GRC I和正常肾小管细胞系HK 2中的表达。　结果　死亡受体DR4、DR5在肾癌组织及正常肾组织 ,肾癌细胞系GRC I和正常肾小管细胞系HK 2中强表达 ;假受体DcR 1在正常肾组织和正常肾小管细胞系HK 2中强表达 ;假受体DcR 2未见表达。　结论　TRAIL基因在肾癌肿瘤细胞的凋亡机制中可能发挥重要的作用。Objective To investigate the ex pression of tumor necrosis factor related apoptosis inducing ligand (TRAIL) r eceptors in renal cell carcinorma. Methods TRAIL receptors mRNA were tested through RT PCR and Northern Blot in renal cell carcinoma tissue and renal cell carcinoma cell line (GRC I). Results The expression of death receptor 4(DR4) and death recep tor 5(DR5) was certificated in renal cell carcinoma and normal renal cells. Deco y receptor 1(DcR1) expressed only in normal renal tissue and cell line. Decoy re ceptor 2(DcR2) was not detected in normal and tumor tissue and cell line. Conclusion TRAIL gene may play an important role in apoptosis of renal cell carcinoma.

关 键 词：肿瘤坏死因子 配体 肾肿瘤 细胞凋亡 TRAIL 

分 类 号：R737.11[医药卫生—肿瘤]