应用大鼠中期肝癌试验研究DEN和2-AFF的促癌作用  

Cancer promoting effects of N-nitrosodiethylamine (DEN) and 2-acetylaminofluorene (2-AAF) with medium term rat liver bioassay

作　　者：顾性初[1] 钱蓓丽[1] 张慧娟[1] 蒋建强[1] 叶慧英[1] 朱玉珍[1] 王吉成[1] 

机构地区：[1]上海医药工业研究院,上海200437

出　　处：《中国药理学通报》2001年第3期289-292,共4页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展规划项目!;NoG19980 5 1119

摘　　要：目的　应用大鼠中期肝癌试验研究二乙基亚硝胺(DEN)和 2 乙酰氨基芴 (2 AAF)的促癌作用。方法　♂SD大鼠单次ip 2 0 0mg·kg-1DEN启动 ,2wk后每天在饮水中加入 10、33、10 0 ppmDEN ,或以 2 2、6 6、 2 2mg·kg-12 AAF灌胃 ,连续 6wk。第 3周全部大鼠切除 2 /3肝 (partialhepatectomy) ,第 8周末处死 ,以免疫组化方法检测肝脏胎盘型谷胱甘肽S 转移酶 (GST P) ,以GST P阳性灶数量 /面积相对于仅DEN启动的对照组的增加 ,评价化合物的致癌性。结果　DEN和 2 AAF均引起肝GST P阳性灶数量和面积增加 ,并且显示剂量效应关系。结论　DEN和 2 AAF均具有促癌作用 ,大鼠中期肝癌试验是研究受试物促癌作用的简便、经济、有效的方法。AIM To study the cancer promoting effects of N nitrosodiethylamine (DEN) and 2 Acetylaminofluorene (2 AAF). METHODS Medium Term Rat Liver Bioassay (MTRLB). Male SD rats were initially given a single dose (200 mg·kg -1 ) of DEN ip and starting 2 weeks later, were treated with 10, 33 and 100 ppm DEN in drinking water, or with 2 2, 6 6 and 22 mg·kg -1 2 AAF by gavage for 6 weeks. All rats were subjected to two thirds partial hepatectomy at week 3 and killed at the end of week 8. Carcinogenic potential was scored by comparing the numbers and areas in induced glutathione S transferase placental form (GST P) positive foci in the liver with those of corresponding control group given DEN alone. RESULTS Both DEN and 2 AAF caused the increases of the numbers and areas of GST P positive foci in the liver, and showed dose response relationship. CONCLUSION Both DEN and 2 AAF shows cancer promoting effects, and MTRLB was a convenient, economical and effective tool to study the cancer promoting effects of test chemicals.

关 键 词：肝癌 二乙基亚硝胺 2-乙酰所这基芴 促癌作用 

分 类 号：R735.7[医药卫生—肿瘤] R730.231.1[医药卫生—临床医学]