代谢型谷氨酸受体配体(s)-4C3HPG对红藻氨酸诱导性癫痫发作活动的调节研究  

Effect of metabotropic glutamate receptor ligand (s)-4C3HPG on kainic acid-induced seizures and neurotoxicity in rats

作　　者：江文[1] 黄远桂[1] 王洪典[1] 王津存[1] 高华[1] 

机构地区：[1]第四军医大学西京医院神经内科,陕西西安710032

出　　处：《中国药理学通报》2001年第3期323-326,共4页Chinese Pharmacological Bulletin

摘　　要：目的　探讨代谢型谷氨酸受体配体 (s) 4 羧基 3 羟苯基甘氨酸〔(s) 4C3HPG〕对红藻氨酸 (KA)诱导性癫痫发作活动的调节作用。方法　选用KA诱导大鼠复杂部分性发作模型 ,采用侧脑室注射 (s) 4C3HPG法 ,观察其对大鼠癫痫发作行为学、脑电图放电时间以及对大鼠海马的组织病理学影响。结果　 (s) 4C3HPG +KA组大鼠行为学评分与盐水 +KA组相比明显减少 ,在KA注射 40min之后差异有显著性 (P <0 0 1) ,湿狗样运动 (WDS)数目也减少 ,于KA注射后 40、6 0、80、10 0、12 0min差异有显著性 (P <0 0 1)。 (s) 4C3HPG +KA组大鼠EEG虽有癫痫样波发放 ,但放电持续时间较对照组缩短 ,在KA注射 30min之后差异有显著性 (P<0 0 1)。(s) 4C3HPG +KA组大鼠海马CA1、CA3区正常细胞数较对照组大鼠增多 (P <0 0 1) ,死亡或损伤细胞数要减少 (P <0 0 1)。结论　 (s) 4C3HPG可减轻KA诱导性癫痫发作的程度 。AIM To examine the effect of metabotropic glutamate receptor ligand (s) 4 carboxy 3 hydroxyphenyl glycine 〔(s) 4C3HPG〕 on kainic acid induced epileptic seizures and brain damage in rats. METHODS Rats were given (s) 4C3HPG (500 nmol/rat) by icv infusion prior to kainic acid (KA) injection ip(10 mg·kg -1 ). The behavoir changes, electrographic seizure duration and histological alterations between different group of rats were compared. RESULTS (s) 4C3HPG markedly reduced seizures severity in response to kainate. The seizure score in the rat with (s) 4C3HPG injection icv was significantly lower than that of control during the first three hours observation period after KA administration ( P <0 01). The number of wet dog shakes (WDS) was also markedly reduced between 40 and 120 min following KA treatment ( P <0 01). Although the rat treated with (s) 4C3HPG and KA displayed electrographic seizure activity, its seizure duration was significantly shortened. Histopathological analysis showed that treatment with (s) 4C3HPG prevented KA induced neurone loss in the hippocampus to great extent. CONCLUSION (s) 4C3HPG can protect rat against KA induced seizures and neurotoxicity.

关 键 词：代谢型谷氨酸受体 (s)-4C3HPG 癫痫 红藻氨酸 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R971.6[医药卫生—临床医学]