急性运动性轴索型神经病与HLA关联研究  

A study of the association between acute motor axonal neuropathy and HLA.

作　　者：王维平[1] 李春岩[1] 郭力[1] 刘瑞春[1] 王桂荣[1] 龚燕平[1] 曹宁[1] 王纪佐[2] 

机构地区：[1]河北医科大学第二医院神经内科 [2]天津医科大学第二医院神经内科

出　　处：《中国神经精神疾病杂志》2001年第4期250-253,共4页Chinese Journal of Nervous and Mental Diseases

基　　金：国家自然科学基金资助(编号:39470265);河北省自然科学基金资助(编号:399351)

摘　　要：目的 研究急性运动性轴索型神经病（acute　motor　axonal　neuropathy,AMAN）与HLA-Ⅰ类和Ⅱ类等位基因分型的关系,探讨AMAN患者免疫遗传的特点。方法 用改良快速盐析法抽提基因组DNA,采用聚合酶链反应-顺序特异性引物法（PCR-SSP）对33例AMAN患者和132例健康人进行HLA-Ⅰ类和Ⅱ类等位基因分型。结果AMAN组HLA-Ⅰ类基因中B15、B35频率增高,RR值分别为4.09和7.08。Pc（校正P值）分别为0.015和0.00081。AMAN组HLA-Ⅱ类基因中,虽然DR16的频率高于对照组（P=0.004）,但校正后的P值失去意义。结论HLA-B15、B35与AMAN的易感性可能有关联。未发现HLA-Ⅱ类基因与AMAN有明显的关联。Objective To study the relationship between the susceptibility to acute motor axonal neuropathy(AMAN) and frequencies of HLA-class Ⅰ and Ⅱ alleles, and to investigate the characteristics of immune genetic of AMAN. Methods A case-control study was conducted on 33 AMAN and 132 healthy individuals. DNA was extracted from peripheral blood leucocytes by improved fast salt-out technique. HLA-class Ⅰ and Ⅱ gene were typied by DNA-based technology, PCR-sequence specific primers (PCR-SSP) method. Results The frequencies of HLA-B15[relative risk(RR) 4.09,PC 0.015] and HLA-35 (RR 7.08, PC 0.0008) of HLA-class Ⅰ were a significantly increased in patients with AMAN. Frequencies of HLA-DR16 of HLA-class Ⅱ was increased in patients with AMAN ( P = 0.004, but Pc> 0.05) . Conclusions HLA-B15 -B35 might be associated with susceptibility to AMAN. HLA- Ⅱ genes were not found to have any association with AMAN.

关 键 词：急性运动性轴索型神经病 人类白细胞抗原 聚合酶链反应-顺序特异性引物 

分 类 号：R745.43[医药卫生—神经病学与精神病学]