抗真菌抗生素高产菌株的推理选育及其发酵调控研究  

Rational Selection of High Productivity Strains of Antifungal Antibiotic and Studing of Fermentation Condition

作　　者：李淑彬[1] 杨劲松[1] 刘阳[1] 钟英长[1] 陈倩[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2001年第4期13-16,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金资助项目(001214)

摘　　要：探讨海洋霉菌Ｍ１８２菌株对自身次生代谢产物－抗真菌抗生素Ｍ１８２Ａ抗性与该抗生素产量的关系；通过亚硝基胍、亚硝基胍加紫外线复合处理，选育到对该抗生素抗性有明显提高和抗生素发酵相对效价提高到２４４％的高产突变菌株．稳定性实验表明该突变株遗传稳定性良好．在液体发酵中，利用天然有机营养物质发酵效果优于合成化合物．The paper studied the relation between the biosynthesis of antibiolitic antibviolitcs M182A produced by marine mold strain M182 and the resistance of self-secondary metabolites. By UV and UV + NG complex mutation, The high resistance strains against M182A was obtained and whose relative potency increased to 244%. Subculture test indicated that the heredity character of the high productivity strains was stable. The test of liquid fermentation showed natural organ nutrient substances were advantageous to synthesis compounds. Certain temperature shock could improve the yield of M182A.

关 键 词：抗真菌抗生素 自身次生代谢产物抗性 诱变 海洋霉菌 M182A 遗传育种 发酵调控 

分 类 号：TQ465[化学工程—制药化工] TQ460.38