一株假单胞杆菌(Pseudomonassp.)中D-海因酶的分离纯化  

Purification of D-hydantoinase from Pseudomonas 2262

作　　者：石亚伟[1] 李汉卿[1] 袁静明[1] 丁玉华[2] 连惠勇[2] 齐延红[2] 

机构地区：[1]山西大学生物工程中心,山西太原030006 [2]山西省生物研究所,山西太原030006

出　　处：《山西大学学报（自然科学版）》2001年第3期255-258,共4页Journal of Shanxi University(Natural Science Edition)

摘　　要：菌体经超声波处理后 ,上清液首先用热变性作为纯化的第一步 ,后经硫酸铵沉淀和 Q- Sepharose fast flow阴离子交换柱 ,Phenyl- Sepharose fast flow疏水层析 ,Superose12凝胶排阻层析等步骤 ,从 Pseudomonas2 2 6 2菌体中获得电泳纯的海因酶 ,酶活力回收 15 .6 % ,比活为 18.37U/ m g,提纯倍数为 5 9.3。After sonication of the pellets,the supernatant was treated by 55℃ for 15min as the first step of this method.Subsequently,ammonium sulfate fractionation and Q Sepharose fast flow,Pheny1 Sepharose fast flow Superose 12 column chromatography were performed to purify hydantoinase from Pseudomonas 2262 to reach SDS PAGE pure.The analysis of enzymatic activity shown that the activity recovery was 15.6%,specific activity was 18.37U/mg and the purification factor was 59.3 respectively.

关 键 词：Pseudomonas2262 海因酶 纯化 柱层析 假单胞杆菌 酶活力 提纯倍数 

分 类 号：Q814.1[生物学—生物工程] Q939.112