不同浓度亚砷酸对NB_4细胞凋亡的影响  

EFFECTS OF DIFFERENT CONCENTRATIONS OF ARSENOU ACID ON NB_4 CELL APOPTOSIS

作　　者：马东初[1] 孙英慧[1] 马小锋[1] 常奎忠[1] 初俊杰[1] 刘亚革[1] 张素芬[1] 黄世林 

机构地区：[1]沈阳军总医院,沈阳110015 [2]解放军大连中医血液病研究所

出　　处：《肿瘤》2001年第3期162-165,共4页Tumor

摘　　要：目的　研究不同浓度的亚砷酸对NB4 细胞凋亡的影响。方法　采用流式细胞术、DNA电泳和免疫印迹研究测定亚砷酸对NB4 细胞凋亡作用。结果　 (1)经 0 8～ 8μmol·L-1亚砷酸作用 16h后 ,NB4 细胞生存力为 95 0 %以上 ,有“梯状”DNA电泳带和亚二倍体峰 ,细胞在G2 期阻滞 ,但bcl 2表达明显下调 ;(2 )浓度在 10～ 80 μmol·L-1时 ,虽然凋亡广泛发生 ,bcl 2表达明显下调 ,但细胞生存率也降至 31 2 % ;(3)浓度在 40 0 μmol·L-1时 ,细胞生存力为 (80 3± 8 9) % ,无“梯状”电泳带、细胞周期阻滞和bcl 2下调 ,76 6 %细胞被dUTP FITC标记 ,但荧光强度低 ,这也说明在高浓度时 ,亚砷酸诱导凋亡的作用受到了抑制。结论　提示亚砷酸不仅可以诱导NB4 细胞凋亡也可以抑制其凋亡。在低浓度时 ,亚砷酸诱导凋亡的作用强于其抑制凋亡。高浓度时相反。因此 ,通过有机化或化学结构的改变降低其抑制作用可能有助于提高疗效。Objective To study the effects of arsenous acid at different concentrations on NB 4 cell apoptosis. Methods Flow cytometry and DNA gel eletrophoresis and immunoblot were used to determine the degree of NB 4 cells apoptosis. Results 1. After treatment for 16 h with arsenic acid at the concentration of 0.8～8 μmol·L -1 , viability of NB 4 cells was above 95.0% with typical “DNA ladder” on argarose gel, progressive blockage of the cells in G 2 phase, gradual increment of apoptotic NB 4 cells to 48.6% in the presence of bcl 2 expression;2. At the concentration of 10～80 μmol·L -1 , although extensive apoptosis was induced in NB 4 cells and expression of bcl 2 was down regulated, the viability of NB 4 cells significantly reduced to 31.2%. 3. At concentration of 400 μmol·L -1 , the viability of NB 4 cells reincreased to 80.3% without typical “DNA ladder”, cell cycle arrest and down regulation of Bcl 2. Althought 76.6% NB 4 cells were labeled with dUTP FITC the intensity of fluoreseence was very low.Conclusion These results suggest the arsenous acid can not only induce appoptosis of NB 4 cells but also can inhibit its apoptosis. The effects of arsenous acid on the induction of NB 4 cell apoptosis is stronger than its inhibition effects at low doses, while high doses can give a reverse effect. Therefore, low doses of arsenic acid may be helpful to improve the curative effect of leukemia.$$$$

关 键 词：亚砷酸 NB细胞 细胞凋亡 肿瘤细胞 培养 

分 类 号：R73－36[医药卫生—肿瘤]