胃癌组织FHIT基因位点多种遗传不稳定性研究  

MULTIPLE GENETIC INSTABILITY AT FHIT GENE LOCUS OCCURS IN GASTRIC CARCNOMA

作　　者：蔡建春[1] BBapat KPHPritzker 

机构地区：[1]厦门市第一医院肿瘤科及肿瘤研究室,厦门361003 [2]加拿大多伦多大学MountSinai医院

出　　处：《肿瘤》2001年第3期172-173,共2页Tumor

摘　　要：目的　研究胃癌组织中FHIT(fragilehistidinetriad)基因位点的遗传改变。方法　采用放射性同位素PCR为基础的测定方法 ,检测 90例胃癌组织中FHIT基因位点的遗传不稳定性 (GI)。结果　FHIT基因D3S130 0位点GI阳性率为31 1% (2 8/ 90例 ) ,其中微卫星不稳定性 (MSI)、杂合性丢失(LOH)和移码突变 (FS)分别为 11 1% (10 / 90例 )、16 5 %(13/ 79信息例数 )、45 5 % (5 / 11信息例数 ) ,1例同时有MSI和LOH。MSI与胃癌生长方式类型密切相关。结论　上述多种GI表达可能在一定数量胃癌的发生和发展中起重要作用。FHIT基因D3S130 0可以作为检测胃癌GI的重要位点。Objective To investigate the genetic alterations of FHIT gene locus in gastric carcinoma.Methods Genetic instability (GI) was examined in 90 cases of Chinese gastric carcinoma, using a radioisotopic polymerase chain reaction based approach.Results GI at FHIT gene locus was observed in 28(31.1%) of the 90 tumors. The incidences of microsatellite instability (MSI), loss of heterozygosity (LOH) and frameshift (FS) were 11.1% (10 of 90 cases), 16.5% (13 of 79 informative cases), and 45.5% (5 of 11 informative cases) respectively, at FHIT gene D3S1300 locus. Both MSI and LOH were only found in one case. A comparison of GI and the clinicopathological parameters revealed that there was significant correlation between MSI and Ming's different growth patterns of gastric carcinoma.Conclusion These observations suggested that GI is likely to play an important role in development and progress of a significant number of Chinese gastric carcinoma. Therefore, it is also suggested that FHIT gene D3S1300 locus may be an effective key locus to detect GI of human gastric carcinoma.$$$$

关 键 词：胃肿瘤 FHIT基因 胃癌 遗传不稳定性 

分 类 号：R735.2[医药卫生—肿瘤]