9-顺维甲酸对肺鳞癌细胞周期及细胞分化的生物学作用  

Biological Effect of 9-cis-retinoic Acid on Cell Cycle and Differentiation in Human Lung Squamous Carcinoma Cells

作　　者：张世国[1] 孔立新[1] 于雪艳[1] 孙晓明[1] 鲁秀华[1] 

机构地区：[1]解放军济南军区总医院检验科,山东济南250031

出　　处：《临床军医杂志》2001年第2期1-4,共4页Clinical Journal of Medical Officers

基　　金：国家自然科学基金!资助课题 (39770 730 )

摘　　要：目的　观察 9 顺维甲酸 (9 cis RA)对肺鳞癌细胞周期及分化作用的影响。方法　采用流式细胞仪 (FCM )分析 9 cis RA处理前后L78和A2 两肺鳞癌细胞株各周期时相细胞比率 ,同时在光镜下观察用药前后细胞形态与结构的变化。结果　应用 9 cis RA后 ,L78和A2 两肺鳞癌细胞株G0 /G1期细胞比率较对照组显著增高 (P <0 .0 1) ,S期细胞比率显著降低 (P <0 .0 5～ 0 .0 1) ;经 9 cis RA处理后 ,两肺鳞癌细胞株分化特征明显。结论　 9 cis RA对两肺鳞癌细胞株具有抑制细胞分裂、诱导细胞分化的作用。Objective To investigate the effect of 9 cis Retinoic Acid (9 cis RA) on cell cycle and differentiation in L 78 and A 2 Lung squamous carcinoma cell lines. Method Flow cytometry (FCM) was used to detect the rates of G 0/G, S and G 2/M phases cells before and after being treated with 9 cis RA in L 78 and A 2 Lung squamous carcinoma cell lines, and the morphology was also observed. Result Compared with control group, the rates of G 0/G phase cells were higher significantly ( P <0.01), the rates of S phase cells were lower significantly ( P <0.05～0.01) after being treated with 9 cis RA in L 78 and A 2 Lung squamous carcinoma cell lines, and features of cell differentiation were distict in two cell lines. Conclusion The results indicated that 9 cis RA could inhibit cell division and induce cell differentiation in L 78 and A 2 Lung squamous carcinoma cell lines.

关 键 词：9-顺维甲酸 肺肿瘤 细胞周期 细胞分化 肺鳞癌 生物学作用 

分 类 号：R734.2[医药卫生—肿瘤] R730.53[医药卫生—临床医学]