人外周血与骨髓细胞蛋白酪氨酸激酶活性研究  

Study of Protein Tyrosine Kinase Activties in Human Blood and Myelogenic Cells

作　　者：周仲玲[1] 许霖水[2] 杨道理[1] 

机构地区：[1]解放军济南军区总医院免疫科,山东济南250031 [2]第三军医大学,重庆400038

出　　处：《临床军医杂志》2001年第2期34-36,共3页Clinical Journal of Medical Officers

摘　　要：目的　研究人外周血与骨髓细胞蛋白酪氨酸激酶 (PTK)活性 ,为临床诊断、治疗白血病提供基础数据。方法　采用γ 32 P标记及生化方法对正常人及白血病患者的淋巴细胞进行PTK活性测定。结果　外周血PTK活性淋巴细胞白血病各组均高于对照组 ,骨髓PTK活性白血病各组与对照组比较结果差异显著 ,同型白血病外周血与骨髓比较结果亦存在差异。该实验的各组白血病PTK活性质膜高于胞浆。结论　白血病分型不同 ,PTK活性存在差异。该实验可用于临床作为白血病诊断 ,药物疗效观察的指标之一 ,并具有更深入研究白血病发病机制的实用价值。Objective To diagnose and treat leukemia, protein tyrosine kinase (PTK) activities were studied in human blood and myelogenic cell. Method The activities of PTK were measured by gamma- 32 P mark and biochemical method. Result Blood's PTK activities in the lymphocytic leukemia was higher than that in the normal group. PTK activities of myeloplast in the lymphocytic leukemiaes were different. To same type leukemiaes, PTK activity was higher in blood than that in myeloplast. The plasma membrane protein was higher than that in the cytoplasm protein. Conclusion PTK activities are different in types of the leukemia. The experiment may apply one mark to the leukemia diagnosis and treatment.

关 键 词：蛋白酪氨酸激酶 骨髓细胞 淋巴细胞白血病 

分 类 号：R733.7[医药卫生—肿瘤]