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出 处:《水产学报》2001年第3期227-231,共5页Journal of Fisheries of China
基 金:福建省科技项目(重中之重项目)资助
摘 要:用酵母、小球藻、鱼油强化和豆油强化四种不同方式培养轮虫,再分别投喂锯缘青蟹幼体。分析测定轮虫和幼体的生化组成,结果显示,(1)不同方式培养的轮虫之间以及摄食这些轮虫的锯缘青蟹幼体之间的蛋白质含量都没有显著差异;(2)轮虫的脂类含量和脂肪酸组成与培养方式密切相关。小球藻轮虫的脂类含量最高,20:5n-3(EPA)占总脂肪酸的比例也最高,为18.05%。鱼油轮虫则含有最多的22:6n-3(DHA),占总脂肪酸3.16%,脂类含量仅次于小球藻轮虫;(3)锯缘青蟹幼体的脂类含量和脂肪酸组成受相应饵料营养成分的影响。另外,幼体培育实验也发现,饵料营养成分影响幼体的存活率。结果表明,提高轮虫的EPA和DHA含量,尤其是DHA含量,将有利于锯缘青蟹幼体的存活和发育。The rotifers (Brachionus plicalis) were cultivated with four different conditions, fed with yeast (Sacharomyces cerevisiae), the algae (Chlorella sp.), enriched with emulsified preparation of fish oil or soybean oil, and then were fed to larval Scylla serrata. The biochemical composition of rotifers and larvae were examined. The results showed that: (1) There were no significant difference in protein content among rotifers cultivated by different condition and among larvae reared by these rotifers with different nutritional value; (2) The lipid content and fatty acid composition of rotifers were related to culture condition. The algae-fed rotifer (AR) contained the highest lipid content and 20: 5n-3 (EPA, 18. 05% of total fatty acids). The fish oil-enriched rotifer (FR) showed the highest level of 22: 6n-3 (DHA, 3. 16% of total fatty acids) and a lipid content second to the algae-fed rotifer; (3)The lipid content and fatty acid composition of larval Scylla serrata were influenced by those in corresponding rotifers. On the other hand, larvae rearing experiment showed that the nutritional composition in diets influenced the survival rate of larvae. It is suggested that elevation of the content of EPA and DHA in especial will favor the survival and growth of larval Scylla serrata.
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