尼卡地平和表皮生长因子对胶质瘤细胞增殖的影响  

Growth inhibition of epidermal growth factor-stimulated human glioblastoma cells by nicardipine in vito

作　　者：黄垂学[1] 陈兵[1] 张毅夫[1] 胡守兴[1] 

机构地区：[1]中南大学湘雅二医院神经外科,长沙410011

出　　处：《湖南医科大学学报》2001年第3期211-214,共4页Bulletin of Hunan Medical University

摘　　要：目的 :研究钙通道拮抗剂尼卡地平 (Nicardipine ,NC)和表皮生长因子 (EpidermalGrowthFactor,EGF)对恶性胶质瘤细胞系U2 5 1MG生长的作用。方法 :在含 10 %小牛血清和无血清培养基中 ,加入不同浓度的EGF ,NC及NC和EGF(10ng·ml-1)干预 ,应用MTT法观察其细胞生长效应。结果 :在无血清培养基中 ,EGF具有强大的剂量依赖性细胞生长刺激作用 ,10mg·ml-1接近最大效应。加入血清则掩盖EGF的作用。NC能抑制U2 5 1MG细胞的生长 ,在有血清培养基中作用更明显 ;且能完全阻断EGF的生长刺激效应。结论 :NC能抑制胶质瘤细胞的增殖 ,并能完全阻断EGF的生长刺激效应 。Objective To study the growth effects of epidermal Growth Factor (EGF) and the calcium channel antagonist nicardipine (NC) on U251 MG, a human malignant glioma cell lime, which has high-affinity EGF receptors. Methods The growth effects of EGF and NC on U251 MG cultured in serum-free and serum containing (10% fetal bovine serum, FBS) media respectively were observed by MTT colorimeritric analysis. Results (1) EGF significantly enhanced the growth of U251 MG in a dose -dependent manner in serum-free conditons. The near-maximal effect was obtained at 10?mg·ml -1 . The addition of serum obscured this effect. (2) NC decreased U251 MG cells proliferation, especially in serum-containing media, and completely blocked the growth-stimulated effect of EGF. The combinated effects of EGF (10 ng·ml -1 ) and NC equaled those of NC alone. Conclusions When serum is absent U251 MG cells showed a pronounced mitogenic response to EGF in a dosedependent manner, which approximated that achieved with 10% FBS. NC suppressed the growth of U251 MG cells and completely blocked the growth-stimulated effects of EGF, it can be used as an adjuvent therapy for human malignant gliomas.

关 键 词：表皮生长因子 钙通道阻滞剂 尼卡地平 胶质瘤 

分 类 号：R730.264[医药卫生—肿瘤]