HPLC法测定白血病患者血浆中阿糖胞苷及其代谢物浓度  

Determination of Cytarabine and its Metabolite in Plasma of Leukemia Patients by HPLC

作　　者：安富荣[1] 费艳秋[1] 袁静[1] 祝德秋[1] 孙黎[1] 王平全[1] 曹惠明[1] 

机构地区：[1]上海第二医科大学仁济医院临床药学研究室,上海200001

出　　处：《中国临床药理学杂志》2001年第3期228-230,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的：建立测定阿糖胞苷（Ａｒａ－Ｃ）及其代谢产物阿糖尿苷（Ａｒａ－Ｕ）血药浓度的方法，并用于５例急性髓细胞白血病（ＡＭＬ）患者血药浓度监测。方法：采用反相离子对高效液相色谱法，色谱柱： Ｄｉｓｃｏｖｅｒｙ　Ｃ１８柱（５ｍ，２５０ｍｍ × ４．６ｍｍ）；流动相：乙腈－甲醇－０．０１ｍｏｌ·Ｌ－１磷酸盐缓冲液（ｐＨ４．０，含０．０１ ｍｏｌ·Ｌ－１戊烷磺酸钠）（４．５：１．５：９４，ｖ／ｖ／ｖ）；流速：１．0ｍｌ·ｍｉｎ－１；检测波长：２８０ｎｍ．结果：以峰面积外标法定量，线性范围：阿糖胞苷为０．２５-２５ｍｇ·Ｌ－１，阿糖尿苷为１－１００ｍｇ·Ｌ－１。最低检测浓度阿糖胞苷为０．１ｍｇ·Ｌ－１，阿糖尿苷为０．２ｍｇ·Ｌ－１。日内、日间差阿糖胞苷小于９％和１１％，阿糖尿苷小于１％和３％。绝对回收率为９９-１０５％。结论：该方法简便，快速，灵敏度和准确度较高。OBJECTIVE: To develop a method for the determination of Cytarabine (AraC) and its metabolite (uracil arabinoside, Ara-U) in plasma of AML patients. METHODS: A reversed-phase ion-pairing technique utilizing the free amino group of the pyrimidine ring of cytarabine was developed to determine the plasma concentration of cytarabine and Ara-U in patient samples. Chromatographic separation has been achieved on C18 column with acetonitrile-methanol-0.01 mol·L-1 phosphatic buffer (pH4.0, contain 0.01 mol·L-1 l-pentanesulfonate sodium). RESULTS: The standard curve were linear in the range of 0.25 - 25mg.L-1 for cytarabine, 5-l00mg·L-１ for AraU. The minimum detection concentration were 0.lｍg·L-１ for cytarabine, 0.2mg·L-１ for Ara-U. Intra- and inter-day assay precision were less than 9% and １l% for cytarabine, １% and 3% for Ara-U, respectively. Recovery were between 99%-105%. CONCLUSION: The simple method offers accuracy, Precision, sensihvity and efficiency for plasma cytarabine and Ara-U determination.

关 键 词：阿糖胞苷 HPLC法 血药浓度 白血病 药物治疗 

分 类 号：R733.7[医药卫生—肿瘤] R969.1[医药卫生—临床医学]