多次电针刺激抑制大鼠吗啡戒断症状的累加效应及长时程后效应  

CUMULATIVE AND LASTING EFFECTS OF MULTIPLE 100Hz ELECTROACUPUNCTURE STIMULATION SUPPRESSED THE MORPHINE WITHDRAWAL SYNDROME IN RATS

作　　者：吴鎏桢[1] 崔彩莲[1] 韩济生[1] 

机构地区：[1]北京大学神经科学研究所,北京100083

出　　处：《中国疼痛医学杂志》2001年第2期105-108,共4页Chinese Journal of Pain Medicine

基　　金：国家重点基础研究规划"脑功能和脑重大疾病的基础研究"!(G19990 5 40 0 0 )

摘　　要：目的 :观察电针刺激对大鼠吗啡戒断症状的抑制作用是否有累加效应和长时程后效应。方法 :用递增剂量吗啡连续皮下注射 10天 ,造成大鼠吗啡依赖模型。 ( 1)在末次注射吗啡后 12小时 ,将大鼠随机分成 4组 ,分别给予 4次、2次、1次 10 0Hz电针刺激 (每次 30min) ,或不给电刺激作为对照。在末次注射吗啡 2 4小时 ,观察并记录 75分钟内的自然戒断症状。 ( 2 )测试自然戒断症状后 ,将这 4组大鼠继续饲养 7天后 ,用纳络酮 ( 1mg/kg ,i.p .)催瘾 ,观察记录 30分钟内的戒断症状。结果 :( 1) 4次电针刺激组抑制吗啡戒断症状的效果显著优于对照组 (P <0 .0 1)、1次 (P <0 .0 1)及 2次 (P <0 .0 5)电针组。 ( 2 )多次电针刺激后一周 ,仍可以看出电针刺激组纳络酮诱发的戒断症状均显著少于对照组 (P <0 .0 1) ,且 4次电针刺激组的效果显著优于 1次组 (P <0 .0 5)。结论 :( 1) 4次电针刺激抑制自然戒断症状的作用优于单次 ,说明针效有累加作用。 ( 2 )多次 ( 2 - 4次 )电针刺激对戒断症状的抑制作用至少可维持一周。Aim: To observe whether multiple electroacupuncture stimulation (EAS) has a cumulative and lasting suppressing effect on morphine withdrawal syndrome in rats. Methods: (1) Rats were injected twice daily at 08.00 and 20.00 h for 10 days with increasing doses of morphine (10～120 mg/kg s.c.) to make rats dependent to morphine. These rats were then randomly divided into 4 groups (n=10 each group), and EAS (30 min per session) was administered 12 h after the last morphine injection. Group 1 was given 100 Hz EAS 4 times at 08:00, 09:30,18:00 and 19:30 h; Group 2 for 2 times at 08:00 and 19:30 h, and group 3 for once at 19:30 h. Group 4 was kept in the holder without EAS, serving as control. The spontaneous withdrawal syndromes were monitored and scored 24 h after the last injection of morphine for 75 min. (2) Seven days later, naloxone precipitated withdrawal syndromes were monitored and scored for 30 min in the same group of rats. Results: (1) The group receiving 4×EAS expressed less severe spontaneous withdrawal syndrome than the control ( P <0.01), 1×EAS ( P <0.01) and 2×EAS ( P <0.05) groups. (2) The naloxone precipitated withdrawal syndrome of EAS groups tested one week after the first test were significantly reduced compared to the control group ( P <0.01), and the 4′EAS group was significantly better than the 1×EAS group ( P <0.05). Conclusion: (1) Multiple EAS has cumulative effect to suppress morphine withdrawal syndrome (4×EAS > 1×EAS). (2) The effects of multiple (2 4) EAS can last for at least 7 days.

关 键 词：吗啡依赖 戒断症状 电针刺激 累加效应 长效应 大鼠 

分 类 号：R749.61[医药卫生—神经病学与精神病学]