YIGSR五肽及RGD三肽对肝窦内皮细胞窗孔的调节作用  

The Role of YIGSR and RGD Peptides in Regulating Fenestrae of Sinusoidal Endothelial Cells

作　　者：刘小菁[1] 黄明慧[1] 彭雪梅[2] 肖文君[3] 王一平[3] 

机构地区：[1]华西医科大学附属第一医院内科实验室,成都610041 [2]华西医科大学法医学院电镜室,成都610041 [3]华西医科大学附属第一医院消化内科,成都610041

出　　处：《华西医科大学学报》2001年第3期338-340,375,共4页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金资助 (批准号 3 970 0 0 6 8)

摘　　要：目的　探讨酪氨酸 -异亮氨酸 -甘氨酸 -丝氨酸 -精氨酸 (Tyr- Ile- Gly- Ser- Arg,YIGSR)五肽及精氨酸 -甘氨酸 -天门冬氨酸 (Arg- Gly- Asp,RGD)三肽对肝窦内皮细胞 (SEC)窗孔的影响。方法　用胶原酶原位灌注、Percoll不连续密度梯度离心法分离大鼠的 SEC,并加入包被有 型胶原或层粘连蛋白 (lam inin,L N)基质的盖玻片上培养。采用扫描电镜技术及放免方法 ,分别观察 YIGSR五肽及 RGD三肽对 SEC窗孔数目、大小 ,以及 SEC分泌 型胶原能力的影响。结果　生长在包被 L N的盖玻片上 48小时的 SEC,其窗孔数目及大小均比生长在 型胶原上的 SEC明显降低 (P<0 .0 5 ) ;但同时加入 YIGSR五肽 (5 0 μg/ ml)及 RGD三肽 (5 0 μg/ m l)作用 48小时后 ,SEC窗孔数目及大小均有明显增加 (P<0 .0 5 ) ,同样地 ,经 YIGSR五肽及 RGD三肽作用后 ,生长在 L N上的 SEC分泌 型胶原的能力亦明显下降 (P<0 .0 5 )。结论　 YIGSR五肽及 RGD三肽对 SEC的形态 (如窗孔 )及功能 (如分泌 型胶原 )Objective To investigate the effects of Tye Ile Gly Ser Arg (YIGSR) and Arg Gly Asp (RGD) polypeptides on the fenestrae of the liver sinusoidal endothelial cells(SECs). Methods By in situ collagenase perfusion and two step percoll gradient centrifugation, SECs were isolated from normal Wistar rats and cultured on collagen type Ⅰ or laminin coated coverslips. The population of SECs fenestrae and their diameters were observed using scanning electron microscopical technique; and the synthesis of collagen type Ⅳ in SECs was assessed by radio immunoassay (RIA). Results The average number of fenestrae and their diameter in the SECs cultured on laminin coated coverslips were significantly lower than those on collagen type Ⅰ coated coverslips. After the treatment with YIGSR pentapeptides (50ug/ml) and RGD tripeptides (50 ug/ml) together for 48 hrs, the number and diameters of fenestrae in the SECs that grew on laminin coated coverslips increased markedly. Meanwhile, the YIGSR and RGD peptides could significantly decrease the synthesis of collagen type Ⅳ in SECs that grew on laminin coated coverslips. Conclusion The results suggested that the YIGSR and RGD polypeptides may have an important role in regulating the morphology and function of SECs.

关 键 词：酪氨酸-异亮氨酸-甘氨酸-丝氨酸-精氨酸五肽 精氨酸-甘氨酸-天门冬氨酸三肽 肝窦内皮细胞 SEC窗孔 

分 类 号：R364.3[医药卫生—病理学]