A lzheimer病及多梗塞性痴呆患者血清载脂蛋白A、B100、E水平及apoE多态性分析  

Serum Apolipoprotein AⅠ, B100 and E Levels and Apolipoprotein E Polymorphism in Patients with Alzheimer's Disease and Multipleinfarction Dementia in Chinese Population

作　　者：范平[1] 刘宇[1] 张祖辉[1] 刘秉文[1] 葛伟 叶善龙 程吟梅[2] 陈俊杰[4] 

机构地区：[1]华西医科大学基础医学院载脂蛋白研究室,成都610041 [2]解放军杭州疗养院 [3]上海长宁区老年痴呆专科医院 [4]华西医科大学基础医学院重组DNA研究室,成都610041

出　　处：《华西医科大学学报》2001年第3期389-391,共3页Journal of West China University of Medical Sciences

摘　　要：目的　探讨中国人血清载脂蛋白 (apo) AI、B10 0、E水平及 apo E多态性与 Alzheim er病 (AD)及多梗塞性痴呆 (MID)的关系。方法　采用单向免疫扩散法及等电聚焦免疫印迹分析法对 75例 AD、36例 MID和60例对照受检者的血清 apo A 、B10 0、E及 apo E多态性进行了分析。结果　 AD组及 MID组 apo E表型 E4(E4/4 +E3 /4 + E2 /4 )的频率分别为 0 .34 66和 0 .3889,较对照组的 0 .1167明显为高 (P<0 .0 5 ) ,ε4等位基因频率分别为0 .2 333和 0 .2 0 83,均明显高于对照组 (0 .0 666) (P<0 .0 5 ) ,AD组ε2 等位基因频率低于对照组 (0 .0 467vs 0 .0 833,P<0 .0 5 ) ;AD组及 MID组患者血清 apo A 及 E水平明显低于对照组 (P<0 .0 0 1,P<0 .0 5 )。结论　 apo Eε4等位基因与中国人 AD和 MID有关联 ,AD及 MID患者血清 apo A 及 E水平明显降低 (P<0 .0 0 1及 P<0 .0 5 )。Objective To investigate the relationship of serum apolipoprotein (apo)AⅠ, B100 and E levels and apo E polymorphism to Alzheimer's disease (AD) and multiple infarction dementia(MID) in Chinese population. Methods The apoE phenotypes were assayed by isoelectric focusing and immunoblotting, and apolipoproteins were determined by radial immunodiffusion assay in 75 patients with AD, 36 patients with MID and 60 control subjects. Results The frequency of apo E 4 allele (ε 4) was significantly higher and the frequency of ε 2 allele was lower in AD group, compared with those in the control group (0.2333 vs 0 0666, 0.0467 vs 0.0833, P <0.05). The frequency of ε 4 allele in MID group was also higher than that in the control group (0.2083 vs 0 0666, P <0.05). The fasting serum apoAⅠ and E levels in AD and MID groups were remarkably lower than those in the control group ( P <0.001 and P <0.05). Conclusion Apo E 4 allele ε 4 was associated with AD and MID. ApoE4 might be a risk factor for AD and MID, and apo E 2 might be a protective factor for AD. The serum apo AⅠ and apoE levels were significantly decreased in patients with Alzheimer's disease and multiple infarction dementia.

关 键 词：ALZHEIMER病 多梗塞性痴呆 载脂蛋白E 基因多态性 载脂蛋白A1 载脂蛋白B100 

分 类 号：R749.1[医药卫生—神经病学与精神病学]