金属硫蛋白ⅡA过表达对乳腺癌细胞耐药性的影响  

Effects of MT Overexpression on Drug Resisatnce in Breast Cancer Cells

作　　者：张瑞芬[1] 罗贤懋[1] 魏慧娟[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所,北京100021

出　　处：《肿瘤防治研究》2001年第4期284-286,共3页Cancer Research on Prevention and Treatment

摘　　要：目的　研究金属硫蛋白 ( Metallothionein,MT)基因过表达对乳腺癌细胞耐药性的影响。方法　表达质粒 p BAc Neo MT A转染 MCF- 7/Adr细胞 ,用 SRB方法检测了 MT过表达细胞对顺铂的耐药性的变化。结果　与对照细胞相比 ,p BAc Neo MT A转染的细胞对顺铂的耐受性提高 ,其 LD50 由约 1 2 u M增加到大约 30 u M。结论　Objective To study the effects of MT(Metallothionein)overexpression on drug resistance in breast cancer cells.Methods Expression plasmid pBAcNeoMT ⅡA was transfected into breast cancer cell lineMCF 7/Adr cells.The drug resistance of the cells to cisplatin were detected by SRB experiment.Results Compared to the control cells,MCF 7/Adr cells transfected with MTⅡA cDNA showed enhanced resistance to cisplatin,The LD 50 increases from about 12 uM to approximately 30uM.Conclusion Overexpression of MT can increase resistance to cisplatin in breast cancer cells.

关 键 词：金属硫蛋白 乳腺癌细胞株 基因转染 顺铂 乳腺癌 耐药性 

分 类 号：R737.9[医药卫生—肿瘤] R730.53[医药卫生—临床医学]