二氯乙烷对小鼠DNA损伤的器官特异性及时效关系研究  

Studies on the specificity of target organs and repair dynamics of DNA damage induced by 1,2-dichloroethane

作　　者：衡正昌[1] 李爱武[1] 张遵真[1] 

机构地区：[1]华西医科大学公共卫生学院,成都610041

出　　处：《卫生研究》2001年第4期193-195,共3页Journal of Hygiene Research

基　　金：国家自然科学基金 (No.3 9670 62 5 )

摘　　要：为了研究 1,2 二氯乙烷 (DCE)的遗传毒性、探测其致癌的靶器官 ,应用组织匀浆提取细胞核进行彗星试验 ,检测了DCE灌胃染毒 3、8、2 4h后对小鼠肝、肺、肾、脾、骨髓、睾丸、胃、肠、膀胱和外周血淋巴细胞的DNA损伤作用及修复动力学改变。实验结果 :1)DCE在小鼠体内遗传毒性的靶器官是肝、肺、肾、骨髓、结肠及胃粘膜细胞和外周血淋巴细胞 ;2 )DNA损伤和修复的动力学改变在器官间存在较大的差异 ,DNA损伤明显且修复较慢的器官或组织 (肺、胃和血液系统 )与其致癌的靶器官有较好的一致性。提示 :体内多器官的彗星试验对确定体内遗传毒性和预测致癌的靶器官是非常有用的 ;对暴露DCE的生物和人群 ,外周血淋巴细胞在彗星试验的拖尾 ,可作为体内DNA损伤效应的生物标志。In order to investigate the genotoxicity of 1,2 dichloroethane (DCE) and to determine its carcinogenic targets, the DNA damage and repair dynamics were detected by using comet assay in the isolated nuclei with tissue homogenization. Liver, lung, kidney, spleen, bone marrow, testicle, stomach, colon, bladder, and peripheral lymphocytes of mice were isolated at 3h, 8h, 24h after DCE being gavaged. The results showed that: a) liver, lung, kidney, bone marrow, colon, stomach and peripheral lymphocytes were the genotoxic target organs of DCE in mice; b) DNA damage and repair dynamics were obviously different among the test organs, and the organs with DNA damage and slow repaired (lung, stomach and hemocyte) were in accordance with the carcinogenic targets. The results indicate that multiple organ comet assay in vivo is very useful for the evaluation of genotoxicity and prediction of carcinogenic target organ. The comet tail of peripheral lymphocyte is suitable as a biomarker for DNA damage in the target organs of living creatures and population exposed to DCE. It is suggested that in vivo comet assay is also very important to select right marker cells and biomarker in the epidemiological studies on genotoxicity.

关 键 词：二氯乙烷对 DNA损伤 修复 靶器官 彗星试验 时效关系 致癌物 

分 类 号：R730.231[医药卫生—肿瘤]