胰腺癌组织中rasP21,C-erbB-2和P16蛋白的表达意义  

Expressions and Significance of ras P21,C-erbB-2,and P16 Protion in Pancreatic Adenocarcinoma

作　　者：刘香[1] 刘维新[2] 徐永泉[3] 

机构地区：[1]沈阳医学院附属中心医院内科 [2]中国医科大学第二临床学院内科,沈阳110004 [3]中国医科大学第一临床学院消化内科,沈阳110004

出　　处：《中国医科大学学报》2001年第4期287-289,共3页Journal of China Medical University

摘　　要：目的 :分析胰腺癌组织中rasP2 1、C erbB 2和P16蛋白表达的临床意义 ,探讨诊治胰腺癌的有效方法。方法 :4 0例胰腺癌石蜡包埋标本连续切片 5 μm ,HE染色确定肿瘤分化程度 ,应用S P法进行免疫组化染色 ,PBS代替一抗做阴性对照。结果 :rasP2 1和C erbB 2在胰腺癌组织中的阳性率分别为 65 .0 % ( 2 6/ 4 0 )和 4 7.5 % ( 19/ 4 0 )。在高、中、低分化肿瘤中阳性率分别为 5 5 .5 6%、66.67%和 80 .0 0 %及 3 8.89%、5 0 .0 0 %和 60 .0 0 %。经检验无显著性差异 (P >0 .0 5 ) ,但各级阳性表达率呈逐渐增高趋势。P16蛋白在胰腺癌组织中的缺失率为 5 5 % ,在高、中、低分化肿瘤中缺失率分别为 2 7.78%、66.67%和 90 .0 0 % ,经检验有非常显著性差异 (P <0 .0 1)。P16蛋白和rasP2 1、C erbB 2蛋白的表达具有负相关性 ( P <0 .0 5 )。结论 :检测胰腺癌组织中ras癌基因突变及其蛋白产物可作为胰腺癌诊断的分子生物学手段。rasP2 1蛋白和C erbB 2蛋白的表达与肿瘤的恶性度有关 ;P16蛋白的表达可反映胰腺癌的恶性度 ,且同一肿瘤有多基因表达。Objective: Our aim was to find an effective way for diagnosis and treatment of pancreatic adenocarcinoma by analyzing the expressions of rasP21, C erbB 2, and P16 protein in pancreatic adenocarcinoma.Methods: Forty cases of pancreatic adenocarcinoma specimens embedded with paraffin were sectioned serially (5 μm).The HE method was applied to test the differentiation degrees. The expressions of rasP21, C erbB 2, and P16 were detected by immunohistochemical method (S P method). Results: The positive rate of rasP21 and C erbB 2 were 65.0% and 47.5%, respectively. Their positive rate in high, middle, and low differentiated tumor were 55.56%, 66.67%, 80.0% and 38.89%, 50.0%, 60.0%, respectively. No significant difference was found. The positive rate of P16 protein was 45.0%. The frequency of its deletion were 27.78%, 66.67%, and 90.0% in high, middle, and low differentiated tumor. There was very significant difference. The relationship between P16 and rasP21, C erbB 2 protein expression was negative. Conclusion: There are multiple gene expressions in the same tumor. It is a moleculobiological way for diagnosis of pancreatic adenocarcinoma to assay the mutation of ras gene and its product. The expression of P16 protein can reflect the malignancy of tumor.

关 键 词：胰腺癌 RASP21 C-ERBB-2 P16蛋白 免疫组织化学 

分 类 号：R576[医药卫生—消化系统]