CGRP和ET-1在肝纤维化大鼠心肌组织中的表达及意义  

Alterations in the Level of Calcitonin Gene Related Peptide and Endothelin-1 in the Cirrhotic Rat Heart

作　　者：傅华[1] 杨丽[1] 刘小菁[1] 胡宏德[1] 强欧[1] 黄明慧[1] 

机构地区：[1]华西医科大学附属第一医院内科,成都610041

出　　处：《华西医科大学学报》2001年第2期200-201,221,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金!(批准号 3 970 0 0 68);四川省卫生厅基金资助!(批准号 F 0 14 3 )

摘　　要：目的　探讨降钙素基因相关肽 (CGRP)和内皮素 - 1(ET- 1)在肝硬化性心肌病 (CCM)发生中的作用。方法　用放免法分别检测肝纤维化大鼠心肌组织中的 CGRP及 ET- 1分布情况。结果　肝纤维化大鼠组 (n=15 )心脏组织中 CGRP浓度 (74.2 130± 10 .3776 pg/ mg蛋白 )和 ET- 1浓度 (1.4780± 0 .92 35 pg/ m g蛋白 )均显著高于对照组 (n=15 ,P<0 .0 5 ) ;且肝纤维化程度越重 ,心肌组织中 ET- 1浓度增加越明显 (P=0 .0 0 4) ;而心脏组织中CGRP浓度并不随肝纤维化程度的加重而增高 (P=0 .30 7)。结论　心肌 CGRP浓度升高可能系机体对 ET- 1及其他致病因子致心脏功能异常的一种保护 /拮抗反应。在 CCM的病理生理过程中 ,CGRP与 ET- 1这两种体液因素间的平衡紊乱可能具有重要的发病学意义。Objective To investigate the role of calcitonin gene related peptide(CGRP) and endothelin 1(ET 1) in cirrhotic cardiomyopathy (CCM). Methods We measured the level of CGRP and ET 1 in the samples of rat heart collected from 15 liver cirrhosis rats and 15 controls by using radio immunoassay. Results The data showed that the levels of CGRP (74.2130±10.3776pg/mg protein)and ET 1 level(1.4780±0.9235pg/mg protein) were significantly higher in the cirrhotic rat hearts than those in controls ( P <0.05). The increase of ET 1 in the cirrhotic rat hearts was closely associated with the severity of liver cirrhosis ( P =0.004); whereas no significant association was seen between the CGRP concentration and the severity of liver cirrhosis ( P =0.307). Conclusion We infer that the increasing of CGRP level in the cirrhotic rat heart may be a protective or antagonistic reaction to ET 1 or other pathogenic factors for cardiac dysfunction. The disturbance of the balance between CGRP and ET 1 in the liver cirrhosis rat hearts may contribute to the pathologic process of CCM.

关 键 词：肝纤维化 肝硬化性心肌病 降钙素基因相关肽 内皮素-1 大鼠 

分 类 号：R575[医药卫生—消化系统]