Leptin对人成骨细胞增殖和功能的影响  

The Effects of Leptin on Proliferation and Function of Human Osteoblast

作　　者：李双庆[1] 赵红莉[2] 魏松全[2] 安振梅[2] 谢秦[2] 李晓佳[2] 贾卫国[2] 

机构地区：[1]华西医科大学附属第一医院金卡医院,成都610041 [2]华西医科大学附属第一医院内分泌科

出　　处：《华西医科大学学报》2001年第2期240-242,共3页Journal of West China University of Medical Sciences

基　　金：四川省科委科研基金资助!(批准号川科委 1999-10 )

摘　　要：目的　观察瘦素 (leptin)对人成骨细胞增殖和功能的影响。方法　培养人成骨细胞 ,并观察其形态及功能变化。采用 MTT比色法检测不同浓度的 L eptin对人成骨细胞增殖的影响 ,同时观察不同浓度 L eptin条件下骨钙素浓度的变化 ,并用细胞总蛋白较正。结果　体外培养的人成骨细胞大多呈长梭型 ,碱性磷酸酶组织化学染色、骨钙素免疫荧光组织化学染色阳性、培养液中加入 β-甘油磷酸及 L-抗坏血酸后钙茜素红染色阳性。在加入不同浓度 L eptin后第 1、2、3天 ,人成骨细胞增殖无显著变化。L eptin可刺激骨钙素分泌 ,呈浓度依赖性 (P<0 .0 5 ) ,但无时间依赖性 (P>0 .0 5 )。结论　 L eptin对人成骨细胞增殖无影响 ,但可刺激人成骨细胞功能。Objective To observe the effect of leptin on osteoblast. Methods Human osteoblast primary culture was carried out, and the morphology and function of osteoblast were observed. The effects of different levels of leptin on osteoblast in different days were assessed by MTT colorimetry. Osteocalcin production was measured also. Results Human osteoblasts were fusiform in shape and were positive for alkaline phosphatase by histochemical staining, positive for osteocalcin by immunofluorescence staining, and positive by Alizarin Reds staining after mineralized upon supplementation with ascorbate and β glycerophosphate. On the first, second and third days, the prolifertion of osteoblast, cultured with different concentrations of leptin, had no changes. The leptin stimulated synthesis of osteocalcin of cells was found to be dose dependent ( P <0.05), but not time dependent ( P >0.05). Conclusion The above data indicated that there were no evidences for the effects of leptin on the proliferation of human osteoblast, but leptin could enhance the function of human osteoblast.

关 键 词：成骨细胞 瘦素 细胞增殖 骨钙素 LEPTIN 

分 类 号：R589.2[医药卫生—内分泌]