HPLC测定新复方大青叶片中扑热息痛和咖啡因的含量  

Determination of Paracetamol and Caffeine in New compound Folium Isatidis Tablets by HPLC

作　　者：贾善学 郭宇鹏 史保荣 张军华 赵卫 

机构地区：[1]滨州市药品检验所,山东滨州256618

出　　处：《中成药》2001年第5期367-368,共2页Chinese Traditional Patent Medicine

摘　　要：目的 :采用高效液相色谱法同时测定了新复方大青叶片中扑热息痛和咖啡因的含量。方法 :以C18化学键合硅胶为固定相 ,以甲醇 水 (2 5 :75 )为流动相 ,UV检测波长 2 15nm进行测定。结果 :方法的平均加样回收率扑热息痛为 99.6 % ,RSD =0 .6 7(n =5 )咖啡因为 99.3% ,RSD =0 .5 8(n =5 )。扑热息痛在 1.6 μg～ 6 .4μg范围内 ,咖啡因在 0 .16 μg～ 0 .6 4μg范围内 ,浓度与吸收峰面积值呈良好的线性关系。结论 :方法简便、快速 ,结果准确。Objective:To determine the contents of paraceltamol and caffeine in New compouad Folium Isatidis Tablets simultaneously by HPLC. Methods:The determination was carried out with C 18 chemical bonded silica gel as a solid phase, methanol-water (25∶75) as a mobile phase and UV deterction wavelength at 215 nm. Results: The average recovenies of the added sample were 99.6%( RSD=0.67, n=5) for paracetamol and 99.3%(RSD=0.58, n=5) for caffine, There was a good linear relationship between the concentration and absorption area value in the rang of 1.6μg～6.4μg for paractamol or 0.16μg～0.64μg for caffeine.Conclusions: The method is simple, quick and accurate.

关 键 词：HPLC 新复方大青叶片 扑热息痛 咖啡因 中药 含量 

分 类 号：R286.0[医药卫生—中药学]