广东地区妇女子宫平滑肌瘤遗传易感性与HLA-DRB1、DQA1、DQB1相关性的研究  

Association of HLA-DRB1, DQA1, DQB1 with uterine leiomyoma in women in southern China

作　　者：宗利丽[1] 何援利[1] 林江海[2] 刘泽寰[2] 陈为民[2] 潘得京[2] 徐安龙[2] 

机构地区：[1]第一军医大学珠江医院妇产科,广东广州510282 [2]中山大学生命科学院,广东广州510275

出　　处：《第一军医大学学报》2001年第6期436-437,共2页Journal of First Military Medical University

基　　金：国家杰出青年基金(39725007)

摘　　要：目的分析子宫平滑肌瘤与ＨＬＡ－ＤＲＢ１、ＤＱＡ１、ＤＱＢ１等位基因的相关性，从而探讨子宫平滑肌瘤患者的遗传易 感性。方法　用ＰＣＲ－ＳＳＰ及ＰＣＲ－ＳＳＯ技术对５１例外科手术后病理证实为子官平滑肌瘤患者和５０例正常妇女进行 ＨＬＡ－ＤＲＢ１、ＤＱＡ１、ＤＱＢ１等位基因的基因分型。结果　ＨＬＡ－ＤＲＢ１＊０２，ＤＱＡ１＊０６０１在子宫平滑肌瘤组明显高于对照 组（Ｐ＜０．０５，ＲＲ＝５．３７８，１５．４），而ＤＲＢ１＊０１、＊０７，ＨＬＡ－ＤＱＡ１＊０ｌ０２却表现为对照组增高（Ｐ＜０．０５，ＲＲ＝０．２２５，０．３７５， ０．３２９）。结论ＤＲＢ１＊０２、＊０１、＊０７，ＨＬＡ－ＤＱＡ１＊０６０１、＊０１０２基因与子宫平滑肌瘤的遗传易感性相关联。Objective: To study the genetic susceptibility involving the genotypes of HLA-DRB1,DQA1,DQB1 alleies in patients with uterine leiomyoma. Methods The allelic types of HLA-DRB1,DQA1,DQB1 were detected by polymerase chain reaction with sequence-specific primers (PCR-SSP) and polymerase chain reaction-sequence-specific oligonucleotied (PCR-SSO) technique in 51 female patients with utrine leiomyoma at child-bearing age undergoing laparoscopy or surgical operation followed by histological examination. Another 50 healthy and fertile women served as control.Result The frequency of HLA-DRB1 *02, DQA1*0601 in the patient group was markedly higher than that in the control group (P<0.05, RR=5.378,15.4), While the frequency of HLA-DRB1*07, *01; DQA1* 0102 in the control group was higher than that in the patient group(P<0.05, RR=0.225, 0.375, 0.329). Conclusion The results indicate that HLA-DQA1*0601, *0102; DRB1*02, *01,*07 genes may be correlated with the genetic susceptiblity to uterine leiomyoma.

关 键 词：HLA-DR抗原 HLA-DQ抗原 子宫肿瘤 平滑肌瘤 遗传易感性 基因频率 

分 类 号：R737.33[医药卫生—肿瘤]