大鼠脑缺血再灌注后PKCδ表达意义及银杏叶制剂的脑保护作用研究  

Study on the expression of PKC δ after ischemia and reperfusion in rats and the protective effect of Ginaton

作　　者：杨宏[1] 韩雪梅[1] 许吉一[2] 庆疆[2] 王春辉[3] 

机构地区：[1]吉林大学中日联谊医院神经内科,吉林长春130031 [2]长春市中心医院神经内科 [3]吉林省人民医院脑外科

出　　处：《中国老年学杂志》2001年第4期285-287,共3页Chinese Journal of Gerontology

摘　　要：目的　研究蛋白激酶 C(PKC)在大鼠脑缺血再灌注损伤中的表达与细胞凋亡的关系以及银杏叶制剂 (金纳多 )对其表达的影响。方法　用免疫组化法观察 PKC在脑缺血再灌注后不同时间点 PKCδ表达 ,并用图象分析测定其免疫强度以及应用金纳多治疗后对其免疫强度的影响。结果　脑缺血再灌注后 PKCδ阳性表达 ;再灌注 6h PKCδ免疫强度达高峰 ,2 4 h免疫强度减弱 ;金纳多治疗组相应时间点 PKCδ免疫强度明显减弱。结论　 PKC的激活直接参与了缺血性神经元损伤 ;并与细胞凋亡关系密切 ;金纳多抑制 PKC活性 。? Objective To study the relationship between the expression of PKC δ and apoptosis and Ginaton's effect on it.Methods Immunohistochemistry and picture analysis was used to observe the expression change of PKC δ.Results There was expression of PKC δ after ischemia and reperfusion ,and the expression peaked at 6h and decreased at 24h after reperfusion.Ginaton could repress the activation of PKC δ in brain ischemia.Conclusions The activation of PKC directly participate in the injury of neuron after ischemia and reperfusion and might be closely related to apoptosis.Ginaton could repress the activation of PKC,which might be the other mechanism that Ginaton protect brain.

关 键 词：PKC 细胞凋亡 脑缺血再灌注 金纳多 蛋白激酶 银杏叶制剂 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学] R277.733.1[医药卫生—临床医学]