肾上腺切除哮喘大鼠针刺血清的抗哮喘作用研究  

Anti-asthma Effects of Serum Derived from Adrenalectomized Asthma Rats Treated with Acupuncture

作　　者：杨永清[1] 崔龙苹[1] 马淑兰[1] 陈汉平[1] 王宇[1] 金明明[1] 

机构地区：[1]上海市针灸经络研究所,上海200030

出　　处：《针刺研究》2001年第2期114-118,共5页Acupuncture Research

基　　金：国家自然科学基金 (39870 935 );上海市教委(97C0 8)资助项目

摘　　要：在证明针刺对肾上腺切除 (ADX)和普通大鼠哮喘模型具有明显抗哮喘作用的基础上 ,进一步研究了ADX哮喘大鼠经针刺治疗后的血清 (针刺血清 )对上述两种大鼠哮喘模型的作用。结果表明 ,ADX哮喘大鼠经针刺治疗后的血清可明显降低ADX和普通哮喘大鼠的气道阻力与外周血嗜酸粒细胞计数 ,针刺血清注射与针刺治疗的作用之间无明显差别 ,提示针刺血清具有与针刺类似的抗哮喘作用。但针刺血清明显降低两种哮喘大鼠的肺顺应性 ,而针刺则增加其肺顺应性 。Objective: To investigate the effect of serum [derived from adrenalectomized (ADX) asthma rats who accepted acupuncture treatment, “acupuncture serum'] on asthma. Methods: SD rats were randomly divided into ADX + asthma group, asthma group, ADX + asthma + acupuncture group, asthma + acupuncture group, ADX + asthma +serum group, asthma + serum group. “Dazhui' (GV 14), bilateral “Feishu' (BL 13) and “Fengmen' (BL 12) were punctured and the needles were retained for 20 min, which were performed once every other day, 7 sessions altogether. Blood samples were taken to be isolated into serum that was then diluted and injected into the ADX asthma rats via external jugular vein before attack of asthma evoked by egg albumin. Blood eosinophil count was got under microscope after treatment with eosinophil staining solution. Results: In ADX asthma and asthma rats who did not accept acupuncture treatment, after injection of the “acupuncture serum”the airway resistance, pulmonary compliance and eosinophil count were lowered significantly (P<0.05). While after acupuncture treatment, the pulmonary compliance in asthma + acupuncture group increased significantly in comparison with asthma group(P<0.05). The reduction effects of “acupuncture serum' on airway resistance and eosinophil count were the same to those of acupuncture treatment. Conclusion: “Acupuncture serum” has the same anti asthma effects in ADX+asthma rats and asthma rats but their effects on pulmonary compliance are different.

关 键 词：针刺血清 哮喘 肾上腺切除 肺功能 嗜酸粒细胞计数 

分 类 号：R246.1[医药卫生—针灸推拿学] R255.120.5[医药卫生—中医临床基础]