丹参酮对氯化钾及N-甲基-D-门冬氨酸诱导PC12细胞钙超载损伤的保护作用  

Effect of tanshinone and its mechanism on calcium overloading injury in cultured PC12 cells

作　　者：何丽娜[1] 何素冰 杨军[1] 

机构地区：[1]安徽省医学科学研究所省生物医药重点实验室,合肥230061

出　　处：《中国现代应用药学》2001年第3期179-181,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 :探讨丹参酮对 PC12细胞钙超载损伤模型的保护作用及其机制。方法 :采用组织培养法 ,以大鼠肾上腺嗜铬细胞瘤克隆株 PC12细胞为材料 ,制备 KCl及 NMDA诱导的钙超载损伤模型。结果 :形态学检查发现丹参酮对两种不同类型的钙超载损伤模型中的 PC12细胞具有明显保护作用 ,MTT法活细胞测定提示丹参酮可显著提高损伤模型中细胞存活数 ,降低细胞释放的 L DH活性 ,并显著减少胞内钙离子浓度。结论 :丹参酮对钙超载所致OBJECTIVE:To investigate the effect and mechanism of tanshinone on calcium overloading injury models.METHOD:Two injury models induced by KCl and NMDA were used to assay action of tanshinone in cultured PC12 cells.RESULTS:It was found that tanshinone possessed obvious protective effects on PC12 cells in injury models by the way of morphological examination.MTT and LDH measurement in supernate indicated that tanshinone increased number of live cells and reduced the extent of cell injury significantly.Tanshinone also lessened the concentration of calcium ion in cytoplasm.CONCLUSION:Tanshinone protected rat PC12 cells from two calcium over loading injuries effectively in vitro.Its actions may deal with antioxidation,inhibition of NO production and blocking of both types of caloium channel

关 键 词：丹参酮 PC12细胞 钙超载 KCL NMDA 保护作用 

分 类 号：R285.5[医药卫生—中药学]