人眼视神经星形胶质细胞的体外培养及鉴定  

The culture and identification of astrocytes of human optic nerve in vitro

作　　者：郑健樑[1] 张洁[1] 郭彦[1] 文峰[1] 罗燕[1] 

机构地区：[1]中山医科大学中山眼科中心卫生部眼科实验室,广州510060

出　　处：《中华眼底病杂志》2001年第2期144-146,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的　探索人眼视神经星形胶质细胞体外培养方法 ,建立细胞系 ,为进一步研究视神经损伤后的修复提供充足的细胞来源。　方法　用组织块培养法、0 .2 5 %胰蛋白酶和 0 .0 6 % EDTA混合消化液取材培养法 ,对婴儿眼视神经星形胶质细胞进行培养 ,观察细胞形态和生长特性 ,并进行免疫组织化学鉴定。　结果　采用组织块培养的细胞 ,生长较缓慢。用混合消化液培养的细胞 ,贴壁早、生长快 ,约 7d细胞基本融合。培养的细胞胶质纤维酸性蛋白 (glial fibrillary acidic protein,GFAP、S- 10 0蛋白及 Vimentin染色阳性 ,CD34染色阴性。　结论　用混合消化液培养法可成功培养出人眼视神经星形胶质细胞。Objective To culture astrocytes of human optic nerve and establish the cell lines for further study of healing process after optic nerve trauma. [WT5”HZ]Methods [WT5”BZ]Astrocytes of infantile optic nerve were cultured by tissue inoculation or tissue digestion with 0.25% trypsin and 0.06% EDTA. The second and fourth passage cells were stained with HE and anti GFAP, S 100 protein, vimentin, and CD34 antibodies. [WT5”HZ]Results The trypsinized astrocytes of infantile optic nerve reached confluence in 7 days. The cultured cells were in polygonal shape with processes and the cytoplasm was abundant. These cells were positive in GFAP, S 100 protein and vimentin staining, and negative in CD34 staining. Conclusions Astrocytes of human optic nerve can be successfully cultured by trypsinization rather than tissue inoculation.

关 键 词：视神经 星形细胞 细胞培养 体外培养 

分 类 号：R774.6[医药卫生—眼科]