尿素酰基裂解酶酶偶联法测定血清总钙  

A enzymatic method of the determination of calcium in serum

作　　者：蒋兴亮[1] 唐中[1] 张均[1] 

机构地区：[1]川北医学院附属医院检验科,四川南充637000

出　　处：《临床检验杂志》2001年第3期145-146,共2页Chinese Journal of Clinical Laboratory Science

摘　　要：目的　介绍尿素酰基裂解酶酶偶联测定血清总钙的方法。方法　双试剂两点法 ;缓冲液为三乙醇胺 (TEA ,pH 8.0 ,2 0 0mmol/L) ;试剂Ⅰ :含NaHCO3 2 6 .0mmol/L ,NADPH 0 .4mmol/L ,α -酮戊二酸 8 0mmol/L ,尿素酰基裂解酶 115U/L ,GLDH 43 0kU/L ;试剂Ⅱ :含NaHCO3 2 6 0mmol/L ,ATP 6 .2mmol/L。结果　方法线性范围为 0 5～ 5 0mmol/L ,批内和批间平均变异系数分别为 2 13%和 2 6 9%。平均回收率为 10 2 7%。本法 (Y)与邻甲酚酞络合酮法 (OCPC法 ,X1)比较 :r =0 .981,Y1=1.0 2X1-0 .0 2 1;与偶氮胂Ⅲ法 (X2 )比较 :r=0 978,Y2 =0 .99X2 +0 .0 3。血清胆红素高达 30 0 μmol/L ,血红蛋白 4g/L ,镁 2 .5mmol/L ,NH+ 42 0mmol/L ,Trig 8.0mmol/L对本法无明显干扰。结论　本法具有简便、准确、快速 ,适用于自动分析等特点。Objective\ A enzymatic method for measurement of calcium in serum by using urea amidolyase was introduction. Methods \ Double reagent two point method.TEA buffer(pH 8.0,200 mmol/L)was used as the buffer solution.The first reagent consisted of sodium bicarbonate 26.0 mmol/L,NADPH 0.4 mmol/L,2 oxoglutaric acid 8.0 mmol/L,urea amidolyase 115 U/L and GLDH 43.0 kU/L.The second reagent contained sodium bicarbonate 26.0 mmol/L and ATP 6.2 mmol/L in the buffer solution.Results\ The Linearity range was 0.5～5 0 mmol/L.The average rate of recovery was 102.7%.The average within run and between run CV were 2.13% and 2.69%.Camparison with OCPC( X 1),showed that Y 1=1.02X 1-0.021,r=0.981, and with Arsenazo Ⅲ( X 2),Y 2=0.99X 2+0.03,r=0. 978.No interference from as much as 300 μmol/L of bilirubin,4 g/L of hemoglobin,2.5 mmol/L of magnesium,2.0 mmol/L of ammonium ion and 8.0 mmol/L of triglyceride.Conclusion\ This method was simple,accurate and ripid,suitable for automatic analysis of calcium in serum.

关 键 词：钙 酶法 两点法 尿素酰基裂解酶 配偶联法 

分 类 号：R446.112[医药卫生—诊断学]