Effect of Forskolin on Progesterone and Plasminogen Activator Secretion by Perfused Rat Corpora Lutea during Pseudopregnancy  

作　　者：祝诚 赵芳 张丽芳 

机构地区：[1]State Key laboratory of Reproductive Biology,Institute of Zoolog,Academia Sinica,Beijing 100080

出　　处：《Developmental and Reproductive Biology》1993年第1期33-40,共8页发育与生殖生物学报（英文版）

摘　　要：Effects of forskolin on progesterone and plasminogen activator production in pseudopregnant ratcorpora lutea was investigated using isolated in vitro perfused ovaries.Progesterone andplasminogen activator production were measured on day 1,8 and 18 of hCG-inducedpseudopregnancy.The results indicated:different concentrations of forskolin(100,200,400 and800 μg)administered to ovaries on the 8th day of pseudopregnancy caused elevation of progesteronesecretion in a dose-dependent manner.After 8 hours of perfusion,PA contents increasedsignificantly in ovaries treated with forskolin.With exogenous PA-urokinase(800 U)added to theperfusion solution,progesterone secretion increased significantly as compared to control group andremained on high level throughout the perfusion period.Though exerting no apparent effects in lowdosage(5 mM),AMCHA,a PA inhibitor,administered in higher dosage(10 and 15 mM)led tomarked reduction in PA activity and progesterone secretion as compared to control group.Thusforskolin causes significant elevation of level of progesterone secretion and PA activity inpseudopregnant rat ovary perfused in vitro.And PA seems to regulate progesterone secretion in theperfused rat corpora luteum.Effects of forskolin on progesterone and plasminogen activator production in pseudopregnant rat corpora lutea was investigated using isolated in vitro perfused ovaries.Progesterone and plasminogen activator production were measured on day 1,8 and 18 of hCG-induced pseudopregnancy.The results indicated:different concentrations of forskolin(100,200,400 and 800 μg)administered to ovaries on the 8th day of pseudopregnancy caused elevation of progesterone secretion in a dose-dependent manner.After 8 hours of perfusion,PA contents increased significantly in ovaries treated with forskolin.With exogenous PA-urokinase(800 U)added to the perfusion solution,progesterone secretion increased significantly as compared to control group and remained on high level throughout the perfusion period.Though exerting no apparent effects in low dosage(5 mM),AMCHA,a PA inhibitor,administered in higher dosage(10 and 15 mM)led to marked reduction in PA activity and progesterone secretion as compared to control group.Thus forskolin causes significant elevation of level of progesterone secretion and PA activity in pseudopregnant rat ovary perfused in vitro.And PA seems to regulate progesterone secretion in the perfused rat corpora luteum.

关 键 词：FORSKOLIN PROGESTERONE Plasminogen activator Corpus luteum 

分 类 号：Q492.5[生物学—生理学] R973.2[医药卫生—药品]