皮质酮对大鼠延髓头端腹外侧区神经元活动的影响  

Effects of corticosterone on the activity of the neurons in the rostral ventrolateral medulla of rats

作　　者：王伟忠[1] 戎伟芳[1] 王超美[1] 王建文[1] 王继江[1] 袁文俊[1] 

机构地区：[1]第二军医大学生理学教研室,上海200433

出　　处：《生理学报》2001年第4期270-274,共5页Acta Physiologica Sinica

基　　金：theNationalBasicProgramofChina (No G2 0 0 0 0 5 690 5 )andtheNationalNaturalScienceFundationofChina (No 30 0 70 30 6)

摘　　要：实验用多管微电极细胞外记录氨基甲酸乙酯麻醉的SD大鼠延髓头端腹外侧区 (RVLM)神经元的活动。用电刺激主动脉神经和静脉注射苯肾上腺素激活压力感受器反射等方法鉴定心血管神经元 ,在RVLM内共记录到14 5个自发放电的神经元 ,其中 3 3个为心血管神经元 ,3 1个为伤害调制性神经元 ,81个为未知功能神经元。 3 3个心血管神经元微电泳硫酸皮质酮 (CORT)后 ,2 5个 ( 76% )神经元放电迅速加快 ,8个 ( 2 4 % )自发放电没有变化。伤害刺激引起兴奋的 3 1个伤害调制性神经元 ,微电泳CORT后 19个 ( 64% )神经元放电抑制 ,而 2个 ( 6% )兴奋 ,其余 10个 ( 3 0 % )没有反应。功能不明的 81个神经元在微电泳CORT后 ,3 2个 ( 4 0 % )兴奋 ,5个 ( 6% )抑制 ,4 4个( 5 4 % )没有反应。以上结果证明CORT可能通过非基因组机制快速影响RVLM神经元的活动 ,提示在应激等情况下CORT的快速作用机制可能在心血管和抗伤害等活动整合中具有一定意义。Spontaneous discharges of the rostral ventrolateral medulla (RVLM) neurons were extracellularly recorded by multi barrelled microelectrodes in urethane anesthetized rats. A total of 145 neurons were recorded: 33 were cardiovascular, 31 were nociceptive modulating, and 81 were unknown functional. The cardiovascular neurons were electrophysiologically identified through activating baroreceptor reflex by electrical stimulation of the aortic nerve and by intravenous injection of phenylephrine. Of the 33 cardiovascular neurons, 25 (76%) increased in firing rate after iontophoretical application of corticosterone sulfate (CORT), and 8 (24%) failed to respond. Of the 31 nociceptive modulating neurons excited by noxious stimulation, the firing rate decreased in 19 (64%), increased in 2 (6%), and did not respond in 10 (30%) after iontophoresis of CORT. Of the remaining 81 unknown functional neurons, 32 (40%) were excited, 5 (6%) inhibited, and 44 (54%) were not affected by CORT. These results demonstrate that CORT may modulate the activities of the neurons in RVLM through fast non genomical effect, suggesting that the mechanism of the fast actions of CORT may play an important role in integration of the cardiovascular, nociceptive modulating activity under some conditions such as stress.

关 键 词：皮质酮 头端延髓腹外侧区 心血管神经元 伤害调制性神经元 细胞外记录 微电泳 

分 类 号：R363.1[医药卫生—病理学]