卵巢激素对小鼠围着床期子宫内膜Le^y寡糖表达的调控  

Ovary Hormonal Control of Le^y Oligosaccharide Expression during Peri-implantation of Mouse Endometrium

作　　者：王晗[1] 葛常辉[1] 孔英[1] 辛毅[1] 朱正美[1] 

机构地区：[1]大连医科大学生物化学教研室,糖生物学研究所,大连116027

出　　处：《生物化学与生物物理学报》2001年第5期542-546,共5页

基　　金：国家自然科学基金资助项目 ;No .39870 184&&

摘　　要：研究表明Ley 寡糖介导了胚胎与子宫内膜之间的识别与粘附 ,在胚胎植入中起重要作用。其α1,2、α1,3岩藻糖基的合成分别与α1,2岩藻糖基转移酶 (FUT1)、α1,3岩藻糖基转移酶 (FUT4)的催化作用密切相关。应用Western印迹、免疫组化和半定量RT PCR方法 ,观察小鼠妊娠早期、去卵巢后雌孕激素处理的子宫内膜Ley 寡糖抗原以及其合成相关的FUT1、FUT4基因的表达 ,分析卵巢激素对Ley 寡糖表达的调控。结果显示 :妊娠早期 ,FUT1、FUT4基因的转录水平随孕激素水平上升而呈下降的趋势 ,这与Ley 寡糖抗原表达一致。进一步观察发现 ,去卵巢后经孕激素处理 ,FUT1、FUT4基因及Ley 寡糖抗原表达均较对照组降低 ,雌激素处理组表达则明显升高 ,雌孕激素联合作用介于雌激素组和孕激素组之间。结果表明 ,孕激素能下调FUT1、FUT4基因的表达 ,雌激素对其有上调作用 ,两种激素之间表现为相互拮抗。提示雌孕激素可能通过FUT1、FUT4基因转录水平调控LeyIt has been reported that oligosaccharide Le y plays an essential role at the initial stage of implantation. It may act as a mediator molecule for recognition and adhesion between the surface of blastocysts and epithelial cells. Alpha 1,2 fucosyltransferase (FUT1) and α1,3 fucosyltransferase (FUT4) are responsible for the synthesis of its α1,2 and α1,3 fucose, respectively. In this article, the transcription of FUT1 and FUT4 genes was investigated by semi quantitative RTPCR and the expression of Le y oligosaccharide was analysized by Western blotting and immunohistochemistry with the endometrium of early pregnancy mice and of the mice treated by ovary hormones after ovariectomy, in order to investigate the regulation of ovary hormones on the Ley oligosaccharide antigen expression. The results showed that the transcription level of FUT1 and FUT4 genes decreased during early pregnancy through days 1—5, which was in agreement with the increasing level of progesterone. In the ovariectomized group, the transcription level of the genes decreased significantly after progesterone treatment and increased after estrogen treatment, and was at the intermediate level in the group treated with both hormones. The biosynthesis of Ley oligosaccharide paralleled with the transcription ofFUT1 and FUT4 gene. These results suggest that the expression of Ley oligosaccharide in the endometrial epithelium of mice is regulated at the level of transcription of FUT1 and FUT4 genes. It is suppressed by progesterone and stimulated by estrogen, and the effects of both hormones are in antagonism.

关 键 词：Le^y 岩藻糖基转移酶 子宫内膜 激素调节 围着床期 

分 类 号：Q579[生物学—生物化学]