木黄酮对人催乳素瘤细胞增殖及其凋亡影响的实验研究  

EXPERIMENTAL STUDIES OF GENISTEIN ON THE PROLIFERATION AND APOPTOSIS OF HUMAN PROLACTINOMA CELLS CULTURED IN VITRO

作　　者：迟素敏[1] 李承新[2] 朱运龙[1] 刘亚莉[1] 顾建文[1] 陈建康[1] 

机构地区：[1]第四军医大学基础部生理学教研室 [2]西京医院皮肤科

出　　处：《中国应用生理学杂志》2001年第3期209-212,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目 (3 0 0 0 0 14 8)

摘　　要：目的 :观察木黄酮 (genistein ,GST)对培养的人垂体催乳素瘤细胞增殖和凋亡的影响。方法 :将GST、β 雌二醇(E2 )作用于体外培养的人催乳素瘤细胞 ,测定MTT值及3 H TdR掺入量 ,流式细胞仪测定细胞周期 ,并用TUNEL法观察细胞凋亡情况。结果 :不同浓度的GST可抑制催乳素瘤细胞的增殖 ,并存在着剂量效应 ,10 - 5 mol·L- 1 GST可使G1 期的细胞比例从对照组的 5 5 3%上升为 90 3% ;不同浓度的E2 以剂量依赖方式刺激催乳素瘤细胞的增殖 ,并使G2 期的细胞比例从对照组的 15 6 %上升为 41 8%。GST和E2 的共同作用仍可抑制催乳素瘤细胞的增殖 ,但抑制程度降低。不同浓度的GST均明显促进催乳素瘤细胞的凋亡 ,E2 对GST的促凋亡作用无明显影响。结论 :GST在体外能明显抑制培养人催乳素瘤细胞的增殖 ,并促进其凋亡。E2 可部分拮抗GST的抑制增殖作用 。Aim: To study the influence of genistein (GST) on the proliferation and apotosis of cultured human prolactinoma cells. Methods: MTT method and 3 H TdR incorporation and cell cycle analysis were used to examine the changes of proliferation and DNA synthesis of human prolactinoma cells under influence of GST and β estradiol (E 2). Tdt mediated dUTP nick end labeling (TUNEL) were employed to observe the effect of GST and E 2 on the apoptosis of human prolactinoma cells. Results: In a dose dependent manner, GST of different concentration could significantly inhibit the proliferation of human prolactinoma cells cultured in vitro . GST(10 5 mol/L) could increase the proportion of cells in G 1 phase from 55.3% up to 90.3%. E 2 of different concentration could dose dependently increase the proliferation of human prolactinoma cells. E 2(10 -5 mol/L) could increase the proportion of cells in G 2 phase from l5.6% up to 41 .8%. However,a lower suppressive proliferation of cultured human prolactinoma cells was observed with GST and E 2 together.GST,not E 2,could significantly induce the apoptosis of human prolactinoma cells cultured in vitro .Conclusion: GST inhibits the proliferation,DNA synthesis and cell cycle of cultured human pituitary cells,and induces its apoptosis.E 2 decreases partly the effect of GST on the suppression of proliferation,not apoptotic induction,of human prolactinoma cells cultured in vitro .

关 键 词：木黄酮 催乳素瘤 细胞增殖 细胞凋亡 豆类食品 

分 类 号：R736.4[医药卫生—肿瘤] R730.1[医药卫生—临床医学]