脑缺血/再灌流脑微血管内皮细胞ELAM-1和ICAM-1 mRNA表达的研究  

STUDY ON EXPRSSION OF ELAM-1 AND ICAM-1 mRNA ON MICROVASCULAR ENDOTHELIAL CELLS DURING FOCAL CEREBRAL ISCHEMIA/REPERFUSION

作　　者：王中孝[1] 蔡竖平[1] 徐洁[2] 

机构地区：[1]解放军总医院临床药理药学研究窒,北京100853 [2]山东济宁医学院附属医院神经内科

出　　处：《中国应用生理学杂志》2001年第3期236-239,共4页Chinese Journal of Applied Physiology

摘　　要：目的 :探讨ELAM 1和ICAM 1在局部脑缺血 /再灌流炎性反应过程中的作用。方法 :采用尼龙线栓堵大脑中动脉造成局部脑缺血 /再灌流模型 ,用RT PCR方法检测缺血侧脑组织缺血 /再灌流不同时间点ELAM 1和ICAM 1mRNA的表达。结果 :假手术组脑组织未见ELAM 1和ICAM 1mRNA的表达 ,手术组非缺血侧脑组织仅见少量表达。脑缺血 /再灌流后 1h ,缺血侧脑组织ELAM 1和ICAM 1mRNA的表达量已开始升高 ;再灌流后 3h ,ICAM 1mR NA的上调达高峰 ,而ELAM 1mRNA的上调在缺血 /再灌流后 6h达高峰。且持续至缺血 /再灌流后 48h。结论 :EL AM 1和ICAM 1参与了局部缺血再灌流脑组织损伤的病理过程。二者在白细胞进入缺血区脑组织的病理过程中发挥着重要作用。Aim:To evaluate the role of ELAM 1 and ICAM 1 in the course of inflammatory reactions during focal brain ischemia/reperfusion.Methods: The focal brain ischemia/reporfusion model is carried by occluding middle cerebral artery.The expression of ELAM 1 and ICAM 1 mRNA after ischemia/reperfusion was evaluated with RT PCR.Results: No ELAM 1 and ICAM 1 mRNA were detected in the sham operated cortex and only little in the nonischemic cortex.The expression of ELAM 1 and ICAM 1 mRNA were upregulated at 1 hour,peaked at 6 hour and 3 hour respectively and remained elevated for up to 48 hours after ischemia/reperfusion.Conclusion: ELAM 1 and ICAM 1 participate in brain injury during focal ischemia/reperfusion and both of them play an important role in leukocyte infiltration into the ischemic tissues.

关 键 词：脑缺血 再灌流 内皮细胞白细胞粘附分子1 细胞间粘附分子1 反转录多聚酶链反应 

分 类 号：R743.31[医药卫生—神经病学与精神病学]