转化生长因子β_1基因在胃癌中的表达及其临床意义  

Expression of Transforming Growth Factor β_1 in Gastric Cancer and its Clinical Value

作　　者：卢震亚[1] 黄怀德[1] 厉有名[1] 陈峰[1] 蔡卫民[1] 

机构地区：[1]浙江大学第一附属医院,310003

出　　处：《实用癌症杂志》2001年第4期397-399,402,共4页The Practical Journal of Cancer

摘　　要：目的　研究转化生长因子 β1(TGF β1)mRNA在胃癌组织中的表达及其临床意义。方法　采用RT PCR结合斑点印迹法检测胃癌组织、癌旁组织、肠化生组织和正常对照组织中的TGF β1mRNA水平 ,斑点印迹结果采用IS 10 0 0型多功能凝胶成像仪进行定量分析。结果　胃癌组的TGF β1mRNA水平 (1.48± 0 .17)显著高于正常对照组 (0 .88± 0 .0 8)、癌旁组 (0 .92±0 .11)和癌前病变组 (1.0 0± 0 .14 ) ,P <0 .0 1;早期胃癌组的TGF β1mRNA水平显著高于正常对照组和癌旁组 ,P <0 .0 5 ;进展期胃癌组 (1.5 4± 0 .2 0 )和有淋巴结转移组 (1.62± 0 .2 6)其水平显著高于早期胃癌组 (1.18± 0 .2 0 )和无淋巴结转移组 (1.2 5± 0 .19) ,P <0 .0 5。结论　TGF β1与胃癌的发生、发展有密切关系 ,并与胃癌的浸润和淋巴结转移有关。Objective To examine the expression of transforming Growth Factor β 1(TGF-β 1) in patients with gastric carcinoma and the clinical value.Methods Reverse transcription polymerase chain reaction add Dot blot analysis were used to examine TGF-β 1 mRNA levels in gastric cancer tissue,intenstinal metaplasia and normal stomach tissue.Results The gastric cancer had significantly higher TGF-β 1 mRNA levels(1.48±0.17) compared with normal tissues(0.88±0.08) paracancer tissue(0.92±0.11) and intenstinal metaplasia(1.00±0.14)(P<0.01),while no significant difference was found between normal tissues and atrophic gastritis(P<0.05).TGF-β 1 mRNA levels in early gastric cancer was significantly lower than those in progressive cancer(1.18±0.20 vs 1.54±0.20)(P< 0.05),but higher than normal tissues.The TGF-β 1 mRNA level in group with metastatic lymph nodes was higher than those without metastatic lymph nodes(1.62±0.26 vs 1.25±0.19)(P<0.05).Conclusion TGF-β 1 mRNA levels are associated with the gastric carcinogenesis,also associated with the node metastasis and invasion of gastric cancer.

关 键 词：胃肿瘤 转化生长因子Β1 逆转录-多聚酶链反应 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]