痛稳素碳末端八肽翻转孤啡肽对抗吗啡镇痛的作用  

Reversal by nocistatin of the effect of nociceptin in antagonizing morphine analgesia in mice

作　　者：陈勇[1] 陈鲤翔[1] 陈强[1] 杨顶建[1] 彭雅丽[1] 杨祺 王锐[1] 

机构地区：[1]兰州大学生命科学学院生物化学与分子生物学系,兰州730000

出　　处：《中国药理学通报》2001年第4期383-385,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金(No 2 0 0 72 0 14 );教育部优秀青年教师教学科研奖励计划基金;教育部跨世纪优秀人才培养计划基金资助课题

摘　　要：目的　观察痛稳素碳末端八肽在小鼠脑内对孤啡肽对抗吗啡的镇痛作用的影响。方法　固相多肽合成法合成了痛稳素碳末端八肽 ,用辐射热甩尾法测定痛阈 ,观察 (1)小鼠脑室注射 (icv)孤啡肽对吗啡镇痛作用的影响 ;(2 )小鼠脑室注射 (icv)痛稳素碳末端八肽对小鼠基础痛阈的影响 ;(3)小鼠脑室联合注射 (icv)痛稳素碳末端八肽和孤啡肽对吗啡镇痛作用的影响。结果　孤啡肽可对抗吗啡的镇痛作用 ;痛稳素碳末端八肽本身不影响小鼠的基础痛阈 ,但可逆转孤啡肽对抗吗啡的镇痛作用。AIM To study the effect of icv nocistatin(10～17) on nociceptin in antagonizing morphine analgesia in mice. METHEODS Nocistatin (10～17) was synthesized by solid phase polypeptide synthesis method. The tail flick test was used to assess the pain threshold. To observe (1) the effect of icv nociceptin on morphine analgesia in mice. (2) the effect of nocistatin(10～17) on basic pain threshold in mice. (3) the effect of icv nocistatin(10～17) together with nociceptin on morphine analgesia. RESULTS Nociceptin antagonized morphine analgesia. Nocistatin(10～17) had no significant effect on basic pain threshold, however, it reversed the effect of nociceptin in antagonizing morphine analgesia. CONCLUSION Nocistatin (10～17) can reverse the effect of nociceptin in antagonizing morphine analgesia at supraspinal level.

关 键 词：固相多肽合成 痛稳素碳末端八肽 孤啡肽 吗啡 镇痛 

分 类 号：R971.1[医药卫生—药品] R977.6[医药卫生—药学]