大鼠局灶脑缺血再灌注后IL-6水平变化与神经元损害关系的探讨  

Investigation of the relationship between IL-6 levels and neuronal damage after cerebral ischemic reperfusion in rats

作　　者：解建波[1] 蒋建章[2] 刘广义[1] 石秉霞[1] 陈玉芳[2] 

机构地区：[1]青岛大学医学院脑血管病研究所 [2]山东省临沂市人民医院神经内科

出　　处：《临床神经病学杂志》2001年第4期218-220,共3页Journal of Clinical Neurology

摘　　要：目的　探讨大鼠局灶脑缺血再灌注后白细胞介素 - 6( IL - 6)水平变化与神经元损害的关系。方法将 60只 Wistar大鼠随机分为正常对照组、假手术组、缺血再灌注组。应用线栓法制作大鼠脑缺血再灌注模型 ,采用免疫组织化学方法检测大鼠脑组织 IL - 6的变化 ,用 HE染色观察神经细胞损伤与白细胞浸润情况 ,并与正常对照组及假手术组大鼠比较。结果　 IL - 6在正常对照组大鼠神经元与胶质细胞仅有少量表达 ,缺血再灌注 6小时时 IL - 6表达较正常神经元稍增多但无统计学意义 ( P>0 .0 5 ) ,12小时开始在梗死周围区增高 ,第 3天显著增强 ,以后逐渐下降。结论　 IL -Objective To investigate the relationship between IL 6 levels and neuronal damage after cerebral ischemic reperfusion in rats.Methods 60 Wister rats were divided randomly into the following groups: normal control group, sham operative group and ischemic reperfusion group. Adopting the model of ischemic reperfusion, the change of IL 6 in the rat's brain tissue was determined with the method of immunohistochemistry, and the neuron necrosis and WBC infiltration were observed by HE dye,as compared with the normal control group and sham operative group.Results There was little expression of IL 6 in neuron and gliocytes of the normal control group,normal neuron could express little Il 6.There was no statistical significance between the expression at 6h reperfusion and that of normal group( P >0.05).The expression began to increase around the infarction at 12h reperfusion,it reached the peak at the 3th day in the boundary zone and then it gradually decreased.Conclusion The overexpression of IL 6 played an important role in the course of cerebral ischemic reperfusion, and could damage the brain tissue.

关 键 词：白细胞介素6 脑缺血再灌注 炎症 

分 类 号：R743.3[医药卫生—神经病学与精神病学]