原发性鼻咽癌患者her2基因表达的意义  

Significance of her2 oncogene expression in primary nasopharyngeal carcinoma

作　　者：鄢践[1] 方嬿[1] 焦艳[2] 宋立兵[1] 张林杰[1] 黄必军[1] 梁启万[1] 吴秋良[1] 张昌卿[1] 曾益新[1] 

机构地区：[1]中山医科大学肿瘤防治中心,广州510060 [2]江西中医学院

出　　处：《中华医学杂志》2001年第15期904-906,共3页National Medical Journal of China

基　　金：国家 973项目基金资助项目 (G19980 5 12 0 2 ) ;国家杰出青年B类基金资助项目 (3 982 5 5 11) ;中山医科大学"2 11"重点学科建设基金资助项目 (980 45 )

摘　　要：目的　探讨鼻咽癌her2基因在DNA水平的变化并弄清her2癌蛋白在部分鼻咽癌中过度表达的分子机制。方法　用间期荧光原位杂交 (FISH)和免疫组织化学分析方法对 45例原发性鼻咽癌及其癌旁正常组织her2基因在DNA和蛋白质水平的变化。结果　癌旁正常组织细胞核her2信号为 1.92± 0 .10 ,有 2个her2信号和 2个 17号着丝粒信号核占 75 .5 %± 9.3% ;与之相比 ,45例鼻咽癌中无一例有her2基因拷贝数的变化 ;Her2癌蛋白过表达见于 33% (15 / 45 )。但各年龄、性别和不同临床分期鼻咽癌之间表达差异无显著意义。结论　Her2癌蛋白过表达与her2基因扩增无关 。Objective To investigate the status of her2 oncogene at DNA level a nd the molecular mechanism of overexpression of her2 oncoprotein in part of pati ents with nasopharyngeal carcinoma (NPC). Methods Forty-five cases of PNC tissu e and fifteen cases of normal epithelial tissue adjacent to tumor tissue were ex amined for her 2 signal and centromere 17 signal. Interphase fluorescence in sit u hybridization (FISH) was used to examine the copy number of her 2 and immunohi stochemical (IHC) staining were used to analyze the copy number and protein expr ession of her2. Results There were 1.92%±0.10% her2 signals per nucleus in norm al epithelial tissue adjacent to tumor. 75.52±9.33% of the nuclei contained 2 h er2 signals and 2 centromere 17 signals. No alteration in gene copy number was f ound in cancerous nuclei. However, IHC staining of the 45 tumors with antibody a gainst her2 showed that overexpression of her2 oncoprotein occurred in 33% (15/4 5) of NPC. However, there was no significant correlation between her2 expression and sex, age, clinical staging, and clinical outcome of patients. Conclusion Th e overexpression of her2 in NPC does not relate to her2 gene amplification. Immu nohistochemistry may not be a good measure to examine her2 expression. The mecha nism of her2 oncoprotein overexpression and its biological significance in the d evelopment of NPC remain to be elucidated.

关 键 词：原发性鼻咽癌 HER2基因 荧光 原位杂交 

分 类 号：R739.63[医药卫生—肿瘤]