毫米波辐照诱导鼻咽癌细胞凋亡及分子机制的实验研究  

EXPERIMENTAL STUDY OF APOPTOSIS AND ITS MOLECULAR MECHANISMS OF NASOPHARYNGEAL CARCINOMA CELL INDUCED BY MILLIMETER WAVE IRRADIATION

作　　者：王艳萍[1] 袁淑兰[1] 陈晓禾[1] 杨燕[1] 宋毅[1] 陈念永[2] 

机构地区：[1]华西医科大学附属第一医院肿瘤中心肿瘤研究所,四川成都610044 [2]四川省肿瘤医院放疗中心,四川成都610044

出　　处：《红外与毫米波学报》2001年第4期283-286,共4页Journal of Infrared and Millimeter Waves

基　　金：国家自然科学基金 (编号 3 9770 198)资助项目~~

摘　　要：采用细胞计数、光镜和 FCM等方法检测毫米波对人鼻咽癌 CNE1 细胞增殖、细胞凋亡及相关基因表达的影响 ,结果发现 :波长为 7.1mm,频率为 42 .2 GHz,功率密度为 1m W/ cm2 的毫米波辐照 ,1次 /日 ,30 min/次 ,照射 4次 ,能显著抑制 CNE1 细胞增殖和克隆形成 ;并诱导细胞凋亡 ,光镜下可见到明显的凋亡细胞 ,FCM示凋亡百分比显著增加 .FCM结果表明其增殖抑制作用与 P2 1表达上调及 Cyclin D1 表达下调有关 ,其凋亡诱导机制可能是非P5 3依赖的途径 ,而主要通过上调 c- Myc、下调 Bcl- 2和The effect of millimeter wave radiation on the nasopharyngeal carcinoma cell line CNE1 cell proliferation and apoptosis and the expression of related genes was studied by using cell count, light microscopy and the flow cytometry (FCM). By millimeter wave radiation with 1 mW/cm(2) power density at frequency 42.2GHz and wave length 7.1mm on CNE1 cells for a duration of 30min/day for 4 days, cell proliferation and cloning formation were inhibited markedly, and cell apoptosis was induced. FCM also revealed that the inhibiting effect of CNE1 proliferation by millimeter wave was probably associated with the up-regulation of P21 and the down-regulation of Cyclin D-1. The molecular mechanism of apoptosis inducing may be p53-independent and e-Myc-dependent. The up-regulation of c-Myc and Fas and down-regulation of Bcl-2 may play an important role in the process of apoptosis.

关 键 词：毫米波 鼻咽癌细胞株 凋亡 基因表达 分子机制 

分 类 号：R739.63[医药卫生—肿瘤] R730.5[医药卫生—临床医学]