^(60)Coγ射线照射对培养小鼠骨髓基质细胞IL-6表达的影响及机制研究  

EFFECT OF γ RAY IRRADIATION ON THE EXPRESSION OF INTERLEUKIN-6 GENE IN THE CULTURED BONE MARROW STROMAL CELLS AND ITS MECHANISMS

作　　者：朱波[1] 罗成基[2] 魏永江[2] 郭朝华[2] 邹仲敏[2] 高京生[2] 

机构地区：[1]第三军医大学新桥医院全军肿瘤中心,重庆400037 [2]第三军医大学防原医学教研室,全军复合伤研究所重庆400038

出　　处：《辐射研究与辐射工艺学报》2001年第3期214-218,共5页Journal of Radiation Research and Radiation Processing

基　　金：全军"九五"指令性攻关项目 (P6L0 45 )

摘　　要：观察了60 Coγ射线照射对培养小鼠骨髓基质细胞 (BMSCs)的白细胞介素 - 6 (IL - 6 )mRNA及蛋白表达的影响。采用Dexter方法体外培养小鼠BMSCs,8.0Gy60 Coγ射线照射后用放射免疫法测定培养上清液中IL - 6的浓度 ,应用逆转录聚合酶链式反应 (RT -PCR)的方法检测IL - 6的mRNA表达 ,同时与NF -кB抑制剂PDTC或地塞米松预处理的BMSCs相比较。结果表明 ,培养小鼠BMSCs在正常情况下低表达IL - 6 ,辐射后 2h时其mRNA水平表达升高 ,4h时达峰值 ,之后开始下降 ;其蛋白表达在 2h时开始升高 ,8- 12h时达峰值 ,2 4h时基本恢复至正常水平 ;而BMSCs在受照射前 2h用PDTC或地塞米松预处理后 ,辐射并不引起IL - 6mRNA表达的改变。结果显示 ,60 Coγ射线照射能够激活培养小鼠BMSCs细胞IL - 6基因的转录 ,使细胞分泌IL- 6增多 ,其机制可能与照射引起的NF -кB活化有关。The aim of this study was to investigate the effect of radiation on the expression of interleukin-6 in the bone marrow stromal cells (BMSCs) and to discuss the mechanisms by which the expression of interleukin-6 increased. The BMSCs from normal Kungming mice were cultured in vitro with the method of Dexter. The expression of interleukin-6 on the levels of protein and mRNA were detected by the uses of radio-immunity assay(RIA) and the reverse transcription polymerase chain reaction(RT-PCR) respectively. PDTC and dexamethasone, two different inhibitors of nuclear factor-kappa B, were used as controls. The result of RIA showed that the expression of interleukin-6 were distinctly increased at 1 hour, with its peak at 4 hours post irradiation in the supernatant of culture of murine BMSCs when the BMSCs were irradiated by 8.0 Gy 60 Co γ rays. The results of RT-PCR showed that the expression of IL-6 mRNA reached its peak at 8 hours. After adding NF-kappa B inhibitor in the supernatant before radiation, there was no difference between control group and radiation group in detecting the mRNA of IL-6 using RT-PCR. This study suggested that the transcription and expression of IL-6 were activated by ionizing radiation, which might be due to the reason that ionizing radiation could activate NF-kappa B.

关 键 词：照射 骨髓基质细胞 白细胞介素-6 NF-KB Γ射线 辐射损伤 

分 类 号：R818.023[医药卫生—放射医学] R818.74[医药卫生—临床医学]