食用仙人掌的离体培养及其快速繁殖  

Tissue Culture and High Frequency Propagation of Opuntia milpa alta Haw.

作　　者：陈丽静[1] 潘英[2] 马慧[1] 刘少霞[1] 姜明兰[1] 钟文田[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,沈阳110161 [2]沈阳农业大学生物科学技术学院海城天成产学研基地,海城114200

出　　处：《园艺学报》2001年第4期327-330,共4页Acta Horticulturae Sinica

摘　　要：研究了食用仙人掌离体快繁的若干影响因素。适宜的培养基为 :不定芽诱导 ,MS + 6 BA 3.0mg/L +NAA 0 .1mg/L ;继代和增殖 ,MS + 6 BA 1mg/L +KT 0 .5mg/L +NAA0 .3mg/L ,继代培养 30d ,平均增殖倍数为 8;生根培养基为 1/ 2MS +NAA 0 .1mg/L +IBA 0 .1mg/L。在培养过程中 ,有些外植体膨大 ,并有疏松的淡黄色颗粒状愈伤组织出现 ,从愈伤组织表面分化出芽 ;有些外植体不膨大 ,无愈伤组织出现 ,直接分化出丛生芽。过渡移栽基质为田园土∶珍珠岩∶河沙 =1∶1∶1,试管生根苗根长约 1cm时移栽 ,成活率最高可达 95Effects of several factors such as medium,cutting materials and plant growth regulators on in vitro culture and rapid propagation of Opuntia milpa alta Haw.were investigated.Using the Opuntia milpa alta Haw.stem as explant,a method for high frequency propagation of Opuntia milpa alta Haw.was established.The results of the experiments suggested that the appropriate me dium for different culture stages were:(1)Germination of adventitious buds:MS+6 BA 3.0mg/L+NAA 0.1mg/L. On this medium shoot multiplication occurred at a 8 fold rate after 30 days;(2)Differentiation and subculture of rosette buds:MS+6 BA 1mg/L+KT 0.5mg/L+NAA 0.3mg/L;(3)Rootage:1/2 MS+NAA 0.1mg/L+IBA 0.1mg/L.The shoots could be directly rooted in transplantation medium(soil,vermiculite and sand in equal proportion)with up to 95% survival.

关 键 词：食用仙人掌 植物组织培养 快速繁殖 离体培养 培养基 外植体 

分 类 号：S644.9[农业科学—蔬菜学] S603.6[农业科学—园艺学]