维拉帕米对大鼠脂肪肝热缺血再灌注损伤的保护作用  

Protective effects of verapamil on warm ischemia-reperfusion injury of fatty liver in rats

作　　者：李文岗[1] 张学文[1] 何尔斯泰[1] 刘建华[1] 孙辉[1] 

机构地区：[1]白求恩医科大学第三临床医院普外科,长春市130022

出　　处：《中华肝胆外科杂志》2001年第8期487-489,共3页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的探讨脂肪肝缺血再灌注损伤过程中丙二醛、谷丙转氨酶、内皮素、肿瘤坏死因子的变化,以及维拉帕米对脂肪肝缺血再灌注(I/R)损伤的保护作用。方法通过建立脂肪肝动物模型,观察脂肪肝大鼠在缺血前门静脉压力,缺血前、缺血15min 和30min 再灌注60min 后血清谷丙转氨酶(ALT),肿瘤坏死因子(TNFα),内皮素(ET-1)和肝组织中丙二醛(MDA)的变化,以及维拉帕米对 I/R 损伤的保护作用。结果缺血前及 I/R 损伤后,脂肪肝组大鼠肝组织中 MDA 及血清 ET-1、TNFα、ALT 呈升高趋势,药物组则明显降低。结论 (1)肝脂肪变性可导致肝窦狭窄和不规则,门静脉压升高。(2)含大量脂质的肝细胞对 I/R 损伤敏感性增高,通过产生过多的 MDA,ET-1,TNFα,导致肝细胞破坏,血清 ALT 升高。(3)缺血前应用维拉帕米,对脂肪肝 I/R 损伤起保护作用。Objective To investigate changes in MDA,ALT,ET-1 and TNFα during warm ischemia- reperfusion injury of the liver and explore protective effects of verapamil on the injury.Methods After the model of fatty liver was established in rats,changes in portal vein pressure before ischemia and those in MDA,ALT,ET- 1 and TNFα as well as effects of verapamil before ischemia and ischemia for 15 minutes or 30 minutes followed by 60 minutes of reperfusion were observed.Results Portal vein pressure was significantly higher in the experimental group than in the control.The contents of MDA,ALT,ET-1 and TNFα in fatty liver group before and after the is- chemia were higher than those in the control.These parameters were significantly lower in those rats receiving vera- pamil.Conclusions Liver steatosis can result in narrowing and irregularity of the liver sinus and increase portal vein pressure.Those liver cells containing fat droplets are sensitive to ischemia/reperfusion injury.MDA,TNFα and ET-1 produced by ischemia/reperfusion injury destroy liver cells and increase ALT level.Verapamil can pro- tect ischemia/reperfusion injury of the fatty liver.

关 键 词：再灌流损伤 丙二醛 肿瘤坏死因子 内皮素 维拉帕米 大鼠 脂肪肝热缺血 

分 类 号：R575.5[医药卫生—消化系统]