低剂量电离辐射诱导小鼠胸腺细胞凋亡及细胞周期进程适应性反应的时间效应  

Time-effect of adaptive response of thymocyte apoptosis and cell cycle progression induced by low dose ionizing radiation in mice

作　　者：吕喆[1] 刘淑春[1] 孙祖玥[1] 傅士波[1] 龚守良[1] 

机构地区：[1]吉林大学卫生部放射生物学重点实验室,长春130021

出　　处：《中华放射医学与防护杂志》2001年第4期276-278,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目 (3 9170 2 75 )

摘　　要：目的　观察低剂量辐射诱导胸腺细胞凋亡及细胞周期进程适应性反应的基本规律。方法　实验用X射线照射昆明系雄性小鼠 ,其诱导剂量 (D1 )及其后攻击剂量 (D2 )分别是 75mGy和1 5Gy。D1 和D2 间隔时间分别是 3、6、12、2 4和 6 0h。通过流式细胞仪检测胸腺细胞凋亡和细胞周期进程的变化。结果　当D1 和D2 间隔 3、6和 12h ,D1 +D2 组胸腺细胞凋亡百分数明显低于D2 组(P <0 0 5 ) ,G0 G1 和G2 +M期细胞百分数也不同程度地低于D2 组 ,而S期细胞百分数却明显高于D2 组 (P <0 0 5或P <0 0 1)。结论　上述结果指出 ,D1 在 75mGy ,吸收剂量率为 12 5mGy min ;D2在 1 5Gy ,吸收剂量率为 0 2 87Gy min ;D1 和D2 间隔 3～ 12h ,可在全身照射条件下诱导小鼠胸腺细胞凋亡和细胞周期进程的适应性反应。Objective\ In the present study we observed the general pattern of the adaptive response of thymocyte apoptosis and cell cycle progression induced by low dose radiation. Methods\ Kunming male mice were irradiated with the inductive dose(D 1,75 mGy)and the challenging dose(D 2,1.5 Gy).The intervals between D 1 and D 2 were 3,6,12,24 and 60 hours separately.The changes of thymocyte apoptosis and cell cycle progression were measured with flow cytometry. Results\ When the intervals between D 1 and D 2 were 3,6 and 12 hours,the percentages of apoptotic cells in D 1+D 2 groups were significantly lower than those in D 2 groups ( P< 0.05),and the percentages of G 0/G 1 and G 2+M phase cells decreased in varying degrees,while the percentages of S phase cells increased significantly( P<0.05 or P<0.01) . Conclusion\ The results mentioned above indicate that when the mice were irradiated with 75 mGy(D 1,12.5 mGy/min)3～12 hours before 1.5 Gy(D 2,0.287 Gy/min)exposure,the adaptive response of thymocyte apoptosis and cell cycle progression could be induced under the condition of whole body irradiation.\;

关 键 词：电离辐射 胸腺细胞 细胞凋亡 细胞周期 适应性反应 

分 类 号：R363[医药卫生—病理学]