大鼠中缝大核5-羟色胺神经元——光镜和免疫电镜研究  

SEROTONIN NEURONS IN NUCLEUS RAPHE MAGNUS OF THE RAT: A LIGHT AND ELECTRON MICROSCOPE IMMUNOCYTOCHEMICAL STUDY

作　　者：李戬[1] 范天生[1] 李世东[1] 

机构地区：[1]河南医科大学解剖学教研室

出　　处：《解剖学报》1991年第1期38-42,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金

摘　　要：本研究应用免疫细胞化学PAP法,在光镜和电镜水平,对大鼠中缝大核内5-羟色胺(5-HT)神经元的形态学特征进行了观察。光镜下,5-HT样免疫反应(5-HT-li)神经元为大型多极细胞和梭形细胞,以中缝大核中下部较为密集。在电镜下观察可见:1、5-HT-li产物出现在神经元的胞质和线粒体、粗面内质网、多泡体、囊泡等细胞器的外膜,个别5-HT-li神经元出现核染现象;2、非标记轴突终末和5-HT-li神经元形成轴-体、轴-树、轴-轴突触,非标记轴突终末内多含有清亮圆囊泡(20～30nm),或兼有小颗粒囊泡(40～70nm)、大颗粒囊泡(90～110nm)、清亮扁平囊泡;3、中缝大核内5-HT-li轴突为无髓纤维,5-HT-li轴突终末在中缝大核内分布稀少;4、易见5-HT-li树突和轴突终末邻近毛细血管壁分布;5-HT-li树突可与胶质细胞形成接触。5-HT缝脊神经元在接受多种神经递质系统调节的基础上,可能具有整合作用。本文还对5-HT神经元和脑局部微循环的关系,以及5-HT神经元-胶质细胞关系进行了讨论。Serotonin (5-HT) neurons in the nucleus raphe magnus (NRM) of the rat were identified by light and electron microscope immunocytochemistry methods. Serotoninlike immunoreactive (5-HT-li) neurous were large multipolar and fusiform cells which mainly located in the ventrocaudal part of the NRM. With electron microscopic immunocytochemistry the following findings were revealed: 1. Immunostaining was present in the cytoplasmic matrix, outer membrane of mitochondria, rough endoplasmic reticulum, multivesicular bodies and vesicles. Some nuclei of the cells were stained. 2.5-HT-li somata, dendrites and axons were postsynaptic to numerous unlabeled terminals which contained clear round vesicles (20-30 nm) or mixed with small granular vesicles (40-70 nm), large granular vesicles (90-110 nm) and flat clear vesicles. 3. The 5-HT-li axons were unmyelinated fibers, and the 5-HT-li axon terminals were scarce in the NRM. 4.5-HT-li axon terminals and dendrites abutting on capillaries and 5-HT-li dendrite-glial direct appositions were found in the NRM. These results revealed the ultrastructural characteristics of 5-HT-li neuron in the NRM. The variety of unlabeled terminals making contact with 5-HT-li somata and dendrites suggests that several neuronal system with possibly different transmitters may regulate 5-HT raphe-spinal neurons which may play integrative role in the NRM. Tae relationship between 5-HT neuron and the significance of the local microcirculation and the relationship between 5-HT neurons and glia cells were also discussed in the present paper.

关 键 词：中缝大核 5-羟色胺 免疫细胞化学 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]