B细胞淋巴瘤的Fas蛋白表达及意义  

Fas Protein Expression and its Significance in Patients with Non-Hodgkins B Cell Lymphoma

作　　者：苏祖兰[1] 金亦[1] 唐录英[1] 吴秋良[2] 赵美卿[2] 冯智英[1] 邵春奎[1] 

机构地区：[1]中山医科大学附属第三医院病理科,广东广州510630 [2]中山医科大学附属肿瘤医院病理科,广东广州510060

出　　处：《癌症》2001年第6期612-615,共4页Chinese Journal of Cancer

基　　金：国家自然科学基金资助项目(编号:39870328)

摘　　要：目的：探讨Ｆａｓ蛋白在不同类型 Ｂ细胞源性非霍奇金淋巴瘤（ｎｏｎ－Ｈｏｄｇｋｉｎ’ｓ 　Ｂ 　ｃｅｌｌ 　ｌｙｍｐｈｏｍａ，Ｂ－ＮＨＬ）中的分布及意义，为Ｂ－ＮＨＬ诊治提供新的观察指标及实验依据。方法：对确诊为Ｂ－ＮＨＬ的７９例及良性淋巴组织２０例的石蜡切片，用免疫组化方法进行Ｆａｓ蛋白表达的检测。结果：发现Ｂ－ＮＨＬ的Ｆａｓ蛋白平均阳性表达率为６５．８％，良性淋巴组织为６０．０％，单从数量上，良恶两者之间差异无显著性（Ｐ＜０．０５），而从阳性细胞分布上良、恶性可见区别，良性分布有规律，主要集中在生发中心；恶性分布无规律性。淋巴瘤组内各亚组之间差异有显著性意义：弥漫大Ｂ细胞淋巴瘤（ｄｅｆｆｕｓｅ ｌａｒｇｅ Ｂ ｃｅｌｌ ｌｙｍｐｈｏｍａ， ＤＬＢＬ）组表达分别高于滤泡性淋巴瘤（ｆｏｌｌｉｃｕｌａｒ ｃｅｌｌｌｙｍｐｈｏｍａ， ＦＣＬ）（Ｐ＜０．０５）和小淋巴细胞性淋巴瘤（ｓｍａｌｌ　ｌｙｍｐｈｏｃｙｔｉｃ　ｌｙｍｐｈｏｍａ， ＳＬＬ）组（Ｐ＜０．０１）；ＦＣＬ组表达高于 ＳＬＬ组（ Ｐ＜ ０ ０５）。 Ｆａｓ蛋白的表达与性别。年龄及部位无相关性。结论： １、Ｆａｓ的表达与 Ｂ细胞淋巴瘤的类型及恶性程度有关。２。Objective: The aim of this study was to investigate the distribution and the significance of Fas protein in subtypes of non-Hodgkin's B cell lymphoma (B-NHL), and to provide a new marker for observation and experimental evidence for diagnosis of the patients with B cell lymphoma. Methods: Fas protein expression in paraffin embedded sections of 79 cases conformed B-NHL and of 20 cases benign lymphoid tissue were determined by immunohistochemistry. Results: Fas protein expression was found in 65. 8% of B-NHL and 60. 0% of benign lymphoid tissue. There was no significant difference about quantity between B-NHL group and benign group(P > 0. 05 ), but positive location of Fas protein is different. There were significant differences among three subtypes of B-NHL (P < 0. 05). The expression of Fas protein in diffuse large B cell lymphoma (DLCL) was higher than that in follicular cell lymphoma(FCL) (P < 0. 05) and also than that in small lymphocytic lymphoma (SLL) (P < 0. 01 ). The expression of Fas protein in FCL was higher than that in SLL (P < 0. 05). No significant correlation was found among Fas expression, sex, age, and tumor location (P > 0. 05 ). Conclusions: There may be association in expression of Fas protein among subtypes and grades of B-NHL. The account of Fas expression is not useful for distinguishing benign from malignant groups, but it may be worthful to find locational characteristics of Fas protein in distinguishing benign and malignant lymphoid diseases. It should be valuable to detect quantity and distributuion of Fas protein in conform diagnosis of some difficult cases of B-NHL.

关 键 词：B细胞淋巴瘤 良性淋巴组织 FAS 免疫组织化学 

分 类 号：R733.1[医药卫生—肿瘤]