1364例恶性肿瘤p53蛋白表达的检测及其临床病理学意义  

Immunohistochemical Detection of p53 Protein Alterated Expression in 1364 Patients with Maligant Tumors and Its Clinicopathological Significance

作　　者：卢晓梅[1] 陈朝伦[2] 沈宝茵[2] 

机构地区：[1]新疆医科大学一附院包虫病研究所,新疆乌鲁木齐830054 [2]新疆医科大学一附院病理科,新疆乌鲁木齐830054

出　　处：《癌症》2001年第6期620-623,共4页Chinese Journal of Cancer

基　　金：新疆医科大学第一附属医院科研基金

摘　　要：目的：探讨ｐ５３基因异常表达在人癌发生过程的病理生物学作用和临床病理学意义。方法：对人体１３６４例不同类型恶性肿瘤（其中６１５例鳞癌，３８２例腺癌及３６７例其它恶性肿瘤）及相应良性病变组织进行ｐ５３蛋白表达的免疫组化（ＬＡＳＢ法）检测。结果：恶性肿瘤组织ｐ５３蛋白表达的阳性检出率５６．７％（７７３／１３６４）明显高于良性病变组织 ６． ７％（４２／６２８）（Ｐ＜ ０． ００１）。结论：ｐ５３基因的异常表达（基因突变或／及蛋白积累）是人体多种恶性肿瘤病的普遍现象，可能是人癌发生发展过程中关键的变化之一。免疫组化检测ｐ５３蛋白过度表达可以作为区别良恶性病变有参考价值的指标之一。Objective: The aim of this study was to investigate the effect of p53 gene alterated expression on human carcinogenesis. Methods: p53 protein expression in 1 364 patients with malignant tumors and 628 benign lesions was detected by immunohistochemistry (LSAB). Results: The frenquency of p53 protein positive was significant higher in malignant tumors [56. 67% (733/1364) ] than those in related benign lesions [6. 7% (42/623) (P < 0. 001 ) ]; It was revealed the similar situation as compared squamous cell cancer (615 ), adenocarcinoma (382 ), and other types of malignancies (367 ) with their related benign lesions respectively (P < 0. 001 ). Conclusion: Alterated expression of p53 protein (gene multation or protein accumulation) may involve one of the critical events of human carcinogenesis. Detection of p53 overexpression may be a useful biomarkers for assessment of maligant tumors.

关 键 词：肿瘤 P53基因异常 免疫组化 LASB 临床病理学 

分 类 号：R730.4[医药卫生—肿瘤]