胃癌及癌旁组织中HPV16感染与p21基因失活的相关研究  

The correlative study on infection of humam papilloma virus 16 and p21 genemutation in gastric cancer

作　　者：孙圣荣[1] 张子蕾[1] 姚峰[1] 

机构地区：[1]武汉大学人民医院普外科,430060

出　　处：《中华实验外科杂志》2001年第4期319-320,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　探讨胃癌及癌旁组织中HPV 16的感染及 p2 1基因突变是否存在协同致癌作用及其与胃癌患者预后的关系。方法　采用多聚酶链反应技术检测 46例胃癌及癌旁组织标本中HPV 16型感染 ,并采用免疫组织化学链霉菌抗生物素蛋白 过氧化酶连接法对胃癌 p2 1基因突变进行检测。结果　HPV16阳性率为 41.30 % ( 19/4 6) ,p2 1阳性表达为 5 2 .17% ( 2 4/4 6)。随访表明 46例有 2 1例出现复发或远处转移 ,其中HPV16阳性者复发率为 73.68% ( 14 /19) ,p2 1基因阳性表达者复发率为 66.60 % ( 16/2 4)。结论　HPV 16感染可能是胃癌发生的病因之一 ,而 p2 1基因失活具有协同致癌作用 ,亦可作为判断胃癌预后的指标之一。Objective To explore whether there is a synergistic carcinogensis between the infection of (HPV) and the p21 gene mutat ion in gastric cancer and para-cancerous tissues and their relations with progn osis of the patients with gastric cancer.Methods The infection of HPV16 was detected by using PC R and the p21 gene mutation measured by using immunohistochemical method in the samples of gastric cancer and the para-cancerous tissues from 46 patients with gastric cancer. Results The positive rate of HPV16 was 41.30 % and the g ene mutation rate of p21 was 52.17 %. The recurrence or remote metastasis was ob served in 21 of 46 patients. The recurrence rate was 73.68 % in the patients pos itive for HPV16 and 66.60% in those positive for p21 gene mutation.Conclusion The infection of HPV16 might be one of the causes of gastric cancer genesis and there is syne rgistic carcinogensis in the infection of HPV16 and the p21gene mutation. Both o f them could used as an indicator for evaluating the prognosis of gastric cancer .

关 键 词：胃肿瘤 多聚酶链反应 免疫组织化学 原癌基因蛋白质P21 癌旁组织 临床病理 HPV16 

分 类 号：R735.2[医药卫生—肿瘤] R730.2[医药卫生—临床医学]